苏铁叶的生药学鉴定及抗肿瘤活性部位筛选  

Study on pharmacognosy identification and screening of anti-tumor part from Cyeas revotuta

作　　者：王绍辉[1] 马四补[1,2] 颜昱 程哲康 张亚琛[1] 刘同祥[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,北京100080 [2]贵阳中医学院,贵州贵阳550002

出　　处：《时珍国医国药》2017年第8期1910-1912,共3页Lishizhen Medicine and Materia Medica Research

基　　金：中央民族大学国家级大学生创新创业训练计划资助项目(No.GCYS2016110001)

摘　　要：目的研究苏铁叶的生药学特征及筛选其抗肿瘤活性部位。方法采用原植物形态鉴别、性状鉴别、显微鉴别和薄层色谱鉴别的方法对苏铁叶生药学特征进行鉴定,通过CCK-8法检测苏铁叶二氯甲烷部位、乙酸乙酯部位、正丁醇部位的不同浓度对人肺腺癌细胞A549增殖的影响。结果确定了苏铁叶原植物形态、性状、显微和薄层色谱的主要鉴别特征,发现了苏铁叶二氯甲烷部位、乙酸乙酯部位、正丁醇部位的不同浓度均能不同程度地抑制人肺腺癌细胞A549细胞的增殖。结论苏铁叶的主要鉴别特征可为苏铁叶的生药鉴定、质量标准的制定提供依据,苏铁叶二氯甲烷部位、乙酸乙酯部位、正丁醇部位对人肺腺癌细胞A549具有明显的体外抑制作用,其抑制作用与药物浓度先呈正相关后呈负相关,这为后期对苏铁叶的进一步研究提供理论基础。Objective To study the pharmacognosy identification characteristics and screening of anti-tumor part from Cycas revolute. Methods The original plant morphology,character,microscopic and thin-layer chromatography method of Cyeas revotuta were identified. The effects of dichloromethane extracts,ethyl acetate extracts and n-butanol extracts with different concentrations to act on human lung adenocarcinoma cells A549 by using CCK-8. Results The main distinguishing features of morphology,characters,microscopic and thin-layer chromatography were determined. It was found that different concentrations of dichloromethane,ethyl acetate and n-butanol could inhibit the proliferation of human lung adenocarcinoma cell line A549 in different degrees. Conclusion These characteristics can provide a basis for the pharmacognosy identification,quality standard of Cyeas revotuta. The dichloromethane,ethyl acetate and n-butanol from Cyeas revotuta on human lung adenocarcinoma A549 cells were significantly inhibited in vitro andhave a significant depressant effect on human lung adenocarcinoma cells A549,which relates with its concentration,and this provides a theoretical basis for the further study of Cyeas revotuta.

关 键 词：苏铁叶 生药学 性状鉴别 显微鉴别 抗肿瘤活性部位 

分 类 号：R282.6[医药卫生—中药学]