农杆菌介导的小麦成熟胚愈伤遗传转化研究进展  

Progress in Research on the Genetic Transformation of Wheat Mature Embryo Callus Mediated by Agrobacterium tumefaciens

作　　者：任卉敏 房艳秋 刘玉良[1] 郑术芝[1] REN Huimin FANG Yanqiu LIU Yuliang ZHENG Shuzhi(College of Life Science,Hebei Normal University,Hebei Shijiazhuang 050024,Chin)

机构地区：[1]河北师范大学生命科学学院,河北石家庄050024

出　　处：《河北师范大学学报（自然科学版）》2017年第5期434-439,共6页Journal of Hebei Normal University：Natural Science

基　　金：河北省教育厅自然科学研究项目(QN2015254;QN2015190);河北师范大学科学研究博士基金

摘　　要：小麦是中国重要的粮食作物之一.传统的育种方法存在周期长、改良慢等缺点,基因工程育种现已成为小麦遗传改良的研究热点.因此,小麦成熟胚愈伤组织的遗传转化在小麦遗传改良研究中具有重要意义.农杆菌介导的小麦成熟胚愈伤组织的遗传转化以其自身具有的优势,得到了广泛的关注.介绍了农杆菌介导的小麦成熟胚愈伤遗传转化体系的各种影响因素,如小麦愈伤组织的诱导、分化、转化效率、农杆菌菌株和转化载体等,并对小麦成熟胚愈伤组织遗传转化的发展方向进行了展望.Wheat is one of the significant food crops in China. Since traditional breeding methods have some disadvantages, such as long cycle and slow improvement, genetic engineering breeding has become an important research topic in wheat genetic improvement. Genetic transformation of wheat mature embryo callus is of great significance in the study of genetic improvement of wheat and genetic transformation of wheat mature embryo callus mediated by Agrobacteriurn tumefaciens has attracted attention widely due to its advantages. In this paper, various influencing factors of the genetic transformation system of wheat ma- ture embryo callus mediated by A. tumefaciens, including wheat callus induction, callus differentiation, transformation efficiency,Agrobacterium strains and transformation carriers, etc. In addition,we have fore- seen the prospect of the future direction of genetic transformation of wheat mature embryo callus.

关 键 词：农杆菌 小麦成熟胚 遗传转化 影响因素 

分 类 号：S512.1[农业科学—作物学]