肺腺癌组织与血清中半胱氨酸双加氧酶1基因启动子区甲基化在早期肺癌诊断中的意义  

Detection of cysteine dioxygenase 1 promoter hypermethylation in lung adenocarcinoma using tissue and serum samples

作　　者：陈晨[1] 陆璨[2] 陆前进 

机构地区：[1]中南大学湘雅二医院胸外科,湖南长沙410011 [2]中南大学湘雅医院口腔医学中心,湖南长沙410008 [3]医学表观基因组学湖南省重点实验室,湖南长沙410011

出　　处：《中国医学工程》2017年第8期4-7,共4页China Medical Engineering

摘　　要：目的观察肺腺癌患者肿瘤组织和血清中半胱氨酸双加氧酶1(CDO1)启动子区甲基化状态,探索其与肺腺癌发生进展的关联,及CDO1基因甲基化作为肺腺癌血清学标记物的可能性。方法收集早期肺腺癌患者和肺部良性肿瘤组织及血清样本,采用基于磁珠的DNA提取和亚硫酸氢钠修饰技术,结合实时荧光定量PCR技术检测CDO1基因启动子区甲基化状态。结果肺腺癌组织中CDO1基因甲基化检测率为71.4%(25/35),对应的血清检出率为51.4%(18/35)。对照组中CDO1基因甲基化率为10.0%(2/20),对应的血清检出率为5.0%(1/20),肿瘤组与对照组之间比较差异有统计学意义(P<0.05)。从肺腺癌患者血清检测CDO1启动子区甲基化与从肿瘤组织中直接检测其结果具有很高的一致性(r=0.732,P<0.05)。结论肺腺癌组织和血清DNA中存在CDO1基因启动子区的异常甲基化状态,且具有极高的特异性。血清游离DNA的甲基化检测可能为肺腺癌的早期诊断、复发监测和预后判断提供全新的临床思路。【Objective】To investigate the promoter methylation of cysteine dioxygenase 1（CDO1） in tissue and serum sample from patients with lung adenocarcinoma.【Methods】Tumor tissue samples and serum samples were collected from 35 pathological confirmed lung adenocarcinoma patients and 20 lung benign tumor patients.Followed by real-time polymerase chain reaction,DNA extraction and bisulfite conversion were performed directly on magnetic beads.【Results】CDO1 methylation was detected in 71.4% of the tissue samples and in 51.4% of the serum samples from cancer group,whereas in 10% of the tissue samples and 5% of the serum samples in control group（P〈0.05）.CDO1 promoter methylation detected in serum was consistent with that in tissue samples（r =0.732,P〈0.05）.【Conclusion】CDO1 methylation was detected in tumor and serum more frequently in people with cancer compared to controls.The detection of promoter methylation of CDO1 from serum samples may provide a new clue for the early diagnosis,monitoring,and prognosis evaluation of lung adenocarcinoma.

关 键 词：肺癌 甲基化 DNA 血清 

分 类 号：R563.3[医药卫生—呼吸系统]