右归丸“阴中求阳”配伍对肾阳虚大鼠下丘脑-垂体-肾上腺轴的作用  

Effect of compatibility of reinforcing yang from yin of Yougui pill on hypothalamic-pituitary-adrenal axis in rats with kidney-yang deficiency

作　　者：刘浩龙[1] 王家典[1] 卢鋆[1] 翟鹏云 赵晖[1] 李明[1] 王蕾[1] LIU Haolong WANG Jiadian LU Yun et al(School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, China)

机构地区：[1]首都医科大学中医药学院中医络病研究北京市重点实验室,北京博士研究生100069

出　　处：《环球中医药》2017年第9期1050-1053,共4页Global Traditional Chinese Medicine

基　　金：北京市自然科学基金(7132031);北京市属高等学校高层次人才引进与培养计划-长城学者项目(CIT&TCD20140329)

摘　　要：目的探究右归丸及其拆方滋阴和补阳对肾阳虚大鼠下丘脑-垂体-肾上腺轴的调节作用,阐释右归丸"阴中求阳"理论的科学内涵。方法将30只雄性Wistar大鼠随机分为正常组、模型组、右归丸组、滋阴方组和补阳方组5组,每组6只。采用肌肉注射氢化可的松注射液的方法建立大鼠肾阳虚模型。观察各组大鼠体重、胸腺、肾上腺和脾重量变化;采用放免法测定各组大鼠下丘脑促肾上腺皮质激素释放激素(corticotropin releasing hormone,CRH),血清促肾上腺皮质激素(adreno cortico hormones,ACTH)和皮质酮(Corticosterone,Cort)含量。结果右归丸及其拆方对肾阳虚大鼠的体重、胸腺、肾上腺和脾重量有不同程度的改善作用,并且对大鼠下丘脑CRH、血清ACTH和Cort的含量也有调节作用,而右归丸在调节CRH和Cort方面的作用优于滋阴方。结论右归丸及其拆方均具有改善肾阳虚大鼠的作用,可能与调节肾阳虚大鼠下丘脑-垂体-肾上腺轴有关。右归丸治疗作用优于滋阴方或补阳方。Objectives To study the effect of Yougui pill （YGP） and its disassembled formulas of nourishing yin （NY） and tonifying yang （TY） on regulation of hypothalamic-pituitary-adrenal （HPA） axis in rats with kidney-yang deficiency and illuminate the scientific meaning of reinforcing yang from yin. Methods The 30 Wistar male rats were randomly divided into 5 groups： normal group, model group （MO） , YGP group, NY group and TY group, 6 rats in each group. The kidney-yang deficiency rat model was induced by intramuscular injection with hydrocortisone. The changes of weight, thymus gland, adrenal gland and spleen were observed. The corticotropin releasing hormone （CRH） in hypothalamus, corticosterone （Cort） and adrenocorticotropic hormone （ACTH） in serum were detected by radioimmunoassay. Results The weight of rat, thymus gland, adrenal gland and spleen were improved by YGP and its disassembled formulas. The contents of CRH, ACTH and Cort were regulated by the above formulas. And the effect of YGP in the regulation of CRH and Cort was better than that of BY formula. Conclusion YGP and its disassembled formulas could improve the function of HPA axis in rats with kidney-yang deficiency. It might be related to the regulation of HPA axis. Therapeutical effect of YGP is better than the BY formula or TY formulas.

关 键 词：右归丸及其拆方 肾阳虚大鼠 下丘脑-垂体-肾上腺轴 

分 类 号：R285.5[医药卫生—中药学]