N-乙酰-L-色氨酸对大鼠肝脏缺血再灌注后肠损伤的保护作用  

Protective effect of N-acetyl-L-tryptophan on intestinal damage after rat hepatic ischemia reperfusion

作　　者：王淑惠 姜红心[2] 李晓双[2] 胡梦琪 于海[2] 张明[2] 于树娜[2] 王健欣 蒋吉英[2] Wang Shuhui Jiang Hongxin Li Xiaoshuang Hu Mengqi Yu Hai Zhang Ming Yu Shuna Wang Jianxin Jiang Jiying(Class 17 of Grade 2013,Clinical Medical College ,Wei fang Medical College ,Wei fang ,Shandong 261053,China Teaching and Researching Section of Anatomy ,Wei fang Medical College ,Weifang, Shandong 261053 ,China Class 7 of Grade 2013 ,Clinical Medical College ,Weifang Medical College ,Weifang ,Shandong 261053 ,China)

机构地区：[1]潍坊医学院临床医学院,山东潍坊261053 [2]潍坊医学院人体解剖学教研室,山东潍坊261053

出　　处：《重庆医学》2017年第27期3748-3751,共4页Chongqing medicine

基　　金：山东省自然科学基金资助项目(ZR2010HM006;ZR2014HL020;ZR2014HL021);山东省教育厅资助课题(J11LF14);山东省医药卫生科技发展计划资助课题(2014WS0464);潍坊医学院科技创新研究基金资助项目(K1301001);潍坊医学院大学生科技创新基金资助项目(KX20150091);2015年地方高校国家级大学生创新创业训练计划(201510438010;201510438007)

摘　　要：目的探讨N-乙酰-L-色氨酸(L-NAT)对大鼠肝脏缺血再灌注中肠损伤的保护作用。方法将健康成年雄性SD大鼠24只分为假手术组(Sham组)、缺血再灌注组(IR组)和缺血再灌注加L-NAT组(IR+L-NAT组)。用夹闭肝中叶和左叶肝蒂分支的方法制作肝缺血再灌注模型,用苏木素-伊红(HE)染色法观察小肠组织的形态学结构,用免疫组织化学染色观察激活型Caspase-3、Bax、Bcl-2的表达。结果 (1)IR组小肠绒毛结构破坏,肠黏膜充血脱落,上皮细胞变性坏死,出现炎症细胞浸润;LNAT可使之减轻。(2)免疫组织化学染色显示,与Sham组相比,IR组激活型Caspase-3、Bcl-2和bax的表达升高,L-NAT干预后,Caspase-3和Bax的表达下降,而Bcl-2的表达进一步升高。结论 L-NAT可抑制肝脏缺血再灌注损伤引起的小肠上皮细胞凋亡,减轻小肠上皮细胞损伤。Objective To investigate the protective effects of N-acetyl-L-tryptophan （L-NAT） on intestinal damage after rat hepatic ischemia reperfusion. Methods Twenty four healthy adult rats were divided into the sham operation group （Sham） ,ischemia reperfusion group （IR） ,ischemia reperfusion and N-acetyl-L-tryptophan group（IR＋L-NAT）. The hepatic ischemia reperfusion model was established by occluding the afferent vessels of the left and middle lobes. The morphological structures of the small intes tine were observed by hematoxylin-eosin （HE） staining. The expressions of active caspase-3, Bax and Bcl-2 were detected by immunohistochemistry staining. Results （1） In the IR group, the structure of intestinal villis was destroyed, the intestinal mucosa showed congestion and exfoliation, the epithelial cells had degeneration and necrosis, and infiltration of inflammatory cells appeared; which could be alleviated by L-NAT. （2）The immunohistochemistry showed that compared with the Sham group, the expression of active caspase-3,gcl-2 and Bax in the IR group was increased, after L-NAT intervention,the Bax and caspase-3 expression was decreased,while the Bcl-2 expression was further increased. Conclusion L-NAT could inhibit the apoptosis of small intestinal epithe lial cells caused by liver ischemic reperfusion and attenuates intestinal epithelial damage.

关 键 词：缺血再灌注损伤 小肠 N-乙酰-L-色氨酸 细胞凋亡 大鼠 

分 类 号：R965[医药卫生—药理学]