丙泊酚对发育小鼠海马齿状回神经干细胞的影响  

Effects of propofol on neural stem cells in mouse developing hippocampal dentate gyrus

作　　者：景胜[1] 彭静[1] 包晓航[1] 陈杰[1] 杜智勇[1] 李洪[1] 黄河[1] Jing Sheng Peng Jing Bao Xiaohang Chen Jie Du Zhiyong Li Hong Huang He(Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, Chin)

机构地区：[1]第三军医大学新桥医院麻醉科,重庆400037

出　　处：《重庆医学》2017年第27期3759-3762,3766,共5页Chongqing medicine

基　　金：国家自然科学基金资助项目(81370210)

摘　　要：目的观察丙泊酚对发育小鼠海马齿状回神经干细胞的影响。方法将健康同窝日龄7d(P7)的C57小鼠分为3组:高剂量丙泊酚组、低剂量丙泊酚组和10%脂肪乳对照组。所有小鼠P7接受药物处理。高剂量丙泊酚组腹腔注射丙泊酚60mg/kg;低剂量丙泊酚组腹腔注射丙泊酚30mg/kg;对照组腹腔注射同等体积的10%脂肪乳。部分小鼠注药24h(P8)后处死,其余小鼠饲养至日龄14d(P14)处死。采用免疫组织化学法检测海马齿状回中Ki67、巢蛋白(Nestin)、脑脂结合蛋白(BLBP)及神经元核心抗原(NeuN)的形态及表达量。结果高剂量丙泊酚组P8小鼠海马齿状回中Ki67标记的神经干细胞数量较10%脂肪乳对照组明显减少(P<0.01),且Nestin及BLBP标记的干细胞纤维数量、长度均受损(P<0.05)。P14小鼠海马齿状回中NeuN阳性细胞数量低剂量丙泊酚组与10%脂肪乳对照组比较差异无统计学意义(P>0.05),而高剂量丙泊酚组较10%脂肪乳对照组显著减少(P<0.01)。结论高剂量丙泊酚可抑制海马齿状回神经干细胞的增殖,损伤神经干细胞突起数量及突起形态,导致神经元成熟障碍。Objective To observe .the effects of propofol on neural stem cells in mouse developing hippocampal dentate gyrus （DG）. Methods Healthy 7 d old mice from the same litters were randomly allocated into three groups ; high dose propofol group, low dose propofol group, and 10% fat emulsion control group. All mice were treated with drugs on postnatal 7 d. The mice in high dose propofol group were intraperitoneally injected with 60 mg/kg propofol;the mice in low dose group were intraperitoneally injected 30 mg/kg propofol; while the mice in the control group with equivalent volume of 10% fat emulsion. Some mice were sacrificed at 24 h after medication injection,and the others were sacrificed at postnatal 14 d. The morphology and expression levels of Ki67. Nestin,BLBP and NeuN in hippocampal DG were detected by immunohistochemical method. Results Healthy 7 d old mice from the same litters were randomly allocated into three groups：high dose propofol group,low dose propofol group and 10% fat emulsion control group. All mice were treated with drugs on postnatal 7 d. The mice in high dose propofol group were intraperitoneally injected with 60 mg/kg propofol;the mice in low dose group were intraperitonealty injected 30 mg/kg propofol;while the mice in the control group with equivalent volume of 10% fat emulsion. Some mice were sacrificed at 24 h after medication injection,and the others were sacrificed at postnatal 14 d. The morphology and expression levels of Ki67, Nestin,BLBP and NeuN in hippocampal DG were detected by immunohistochemical method. Conclusion High dose propofol inhibits the proliferation of neural stem cells in hippocampal DG, and impaired the prominence number of neural stem cells and causes neurons dysmaturity.

关 键 词：二异丙酚 干细胞 海马 

分 类 号：R614[医药卫生—麻醉学]