Arabidopsis shaker pollen inward K^+ channel SPIK functions in SnRK1 complex-regulated pollen hydration on the stigma  

作　　者：Dan-Dan Li Huan Guan Fei Li Chang-Zhen Liu Yu-Xiu Dong Xian-Sheng Zhang Xin-qi Gao 

机构地区：[1]State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Tai'an 271018,China

出　　处：《Journal of Integrative Plant Biology》2017年第9期604-611,共8页植物学报（英文版）

基　　金：supported by the Major Research Plan from the Ministry of Science and Technology of China(2013CB945100);the National Natural Science Foundation of China(31270358)

摘　　要：Pollen hydration is a critical step that determines pollen germination on the stigma. KINγ is a plantspecific subunit of the SNF-1-related protein kinase 1 complex （SnRK1 complex）. In pollen of the Arabidopsis kinβγ mutant, the levels of reactive oxygen species were decreased which lead to compromised hydration of the mutant pollen on the stigma. In this study, we analyzed gene expression in kinβγ mutant pollen by RNA-seq and found the expression of inward shaker K^＋ channel SPIK was down-regulated in the kinβγ /pollen. Furthermore, we showed that the pollen hydration of the Arabidopsis spik mutant was defective on the wild-type stigma, althoughthe mutant pollen demonstrated normal hydration in vitro. Additionally, the defective hydration of spik mutant pollen could not be rescued by the wild-type pollen on the stigma, indicating that the spik mutation deprived the capability of pollen absorption on the stigma. Our results suggest that the Arabidopsis SnRK1 complex regulates SPIK expression, which functions in determining pollen hydration on the stigma.Pollen hydration is a critical step that determines pollen germination on the stigma. KINγ is a plantspecific subunit of the SNF-1-related protein kinase 1 complex （SnRK1 complex）. In pollen of the Arabidopsis kinβγ mutant, the levels of reactive oxygen species were decreased which lead to compromised hydration of the mutant pollen on the stigma. In this study, we analyzed gene expression in kinβγ mutant pollen by RNA-seq and found the expression of inward shaker K^＋ channel SPIK was down-regulated in the kinβγ /pollen. Furthermore, we showed that the pollen hydration of the Arabidopsis spik mutant was defective on the wild-type stigma, althoughthe mutant pollen demonstrated normal hydration in vitro. Additionally, the defective hydration of spik mutant pollen could not be rescued by the wild-type pollen on the stigma, indicating that the spik mutation deprived the capability of pollen absorption on the stigma. Our results suggest that the Arabidopsis SnRK1 complex regulates SPIK expression, which functions in determining pollen hydration on the stigma.

关 键 词：Arabidopsis shaker pollen inward K channel SPIK functions in SnRK1 complex-regulated pollen hydration on the stigma 

分 类 号：Q944.4[生物学—植物学]