核因子Ⅰ-C对血小板源性生长因子调节皮肤成纤维细胞转化生长因子β信号通路的抑制作用  

Inhibitory effect of NFI-C on TGF-β signal pathway induced by PDGF in fibroblasts

作　　者：王胜春[1] 李琳[1] 王胜[1] 张嫦娥 宋俐[1] 

机构地区：[1]郑州市儿童医院皮肤科,河南郑州450052

出　　处：《临床皮肤科杂志》2017年第10期691-694,共4页Journal of Clinical Dermatology

摘　　要：目的:探讨核因子(NF)Ⅰ对血小板源性生长因子(PDGF)增强皮肤成纤维细胞转化生长因子β(TGF-β)信号通路的影响作用。方法:构建NFⅠ-C基因慢病毒载体并且确定慢病毒转染成纤维细胞最佳条件,采用Western印迹法测定PDGF对经NFⅠ-C基因病毒转染后成纤维细胞的TGF-β1及其受体Ⅱ(TβRⅡ)蛋白表达的影响作用。结果:(1)NFⅠ-C慢病毒载体转染皮肤成纤维细胞的最佳转染值为50;(2)皮肤成纤维细胞经NFⅠ-C慢病毒转染后NFⅠ-C蛋白的表达较非病毒转染细胞显著升高(P<0.05);(3)正常皮肤成纤维细胞与PDGF共同培养48 h后TGF-β1和TβRⅡ蛋白表达较空白对照显著升高(P<0.05);(4)皮肤成纤维细胞经NFⅠ-C慢病毒转染后与PDGF共同培养后48 h后TGF-β1和TβRⅡ蛋白表达均较正常对照皮肤成纤维细胞和空病毒转染皮肤成纤维细胞显著降低(P<0.05)。结论:血小板源性生长因子可增强皮肤成纤维细胞TGF-β1和TGF-β受体Ⅱ的表达,而NFⅠ-C表达增加可抑制PDGF增强TβRⅡ表达的作用。Objective： To assess the effect of nuclear factor I-C（NFI-C） on transforming growth factor（TGF-13） signal pathway induced by PDGF in fibroblasts. Methods： The lentiviral vector of NFI-C was constructed, and the optimal condition of lentiviral infection was established in fibroblasts. Western blotting was used to assess the effeet of PDGF on expression levels of TGF-β1 and its receptor（TβR Ⅱ ） in fibroblasts transfected with NFI-C. Results： （1） The optimal multiplicity of NFI-C lentiviral infection was 50; （2） Expression levels of NFI-C protein was significantly increased in fibroblasts transfected with NFI-C lentiviral vector compared with that transfected with blank NFI-C lentiviral vector（P〈0.05）; （3） Expression levels of TβR Ⅱ and TGF-β1 were also significantly increased in normal fibroblasts treated with PDGF for 48h compared with that in blank control group（P〈0.05）; （4） Expression levels of TβRlland TGF-β1 were significantly decreased in fibroblasts trans- fected with NFI-C lentiviral vector compared with that in both normal fibroblasts and those treated with blank NFI-C lentivi- ral vector（P〈O.05）. Conclusions： PDGF can increase the expression of TβRⅡ and TGF-β1. Elevated expression levels of NFI-C can inhibit PDGF-indueed increase in TβRⅡand TGF-β1 exoression.

关 键 词：核因子Ⅰ 血小板源性生长因子 皮肤成纤维细胞 

分 类 号：R751[医药卫生—皮肤病学与性病学]