基于基因表达谱分析肾上腺对椎动脉型颈椎病模型大鼠的调控机制研究  

Study of regulation mechanism of adrenal gland on rat model with cervical spondylosis of vretebral artery type based on gene expression profiles

作　　者：宋敏 巩彦龙 刘涛 刘建鸿 董万涛 周灵通 黄凯 侯红艳 

机构地区：[1]甘肃中医药大学中医临床学院,兰州730000 [2]甘肃中医药大学附属医院骨二科,兰州730000

出　　处：《中国临床药理学杂志》2017年第18期1789-1792,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81360554);甘肃省重点中医药科研立项课题基金资助项目(GZK-2010-Z14);甘肃省中药现代制药工程研究院项目开发基金资助项目(2305083306)

摘　　要：目的分析椎动脉型颈椎病(CSA)模型大鼠的肾上腺组织差异基因表达谱变化并探讨肾上腺对CSA的调控机制。方法按照体重将雄性Wistar大鼠随机分为2组:模型组和正常组,用复合造模法建立CSA模型。取模型成功后大鼠的肾上腺组织,提取总RNA,用全基因芯片检测基因表达谱,筛选差异基因,进行基因本体论(GO)、全基因组及代谢途径数据库(KEGG)信号通路分析。结果与正常组比较,模型组差异基因总数为367个(|fold change︱>2,P<0.05),其中表达上调的基因有236个,下调的有131个。富集的GO功能共有2843条,涉及对细胞周期的调节、应力刺激的调节、成纤维细胞增殖的调节及内皮细胞增殖的调节等。参与调节的信号通路共有135条,包括促分裂原活化蛋白激酶(MAPK)信号转导通路、磷脂酰肌醇3激酶/蛋白激酶B(PI3K/Akt)信号通路、Hedgehog信号通路及Wnt信号通路等。结论肾上腺对CSA的调控主要是通过对血管内皮细胞和血管平滑肌细胞的增殖迁移、黏附分子、成纤维细胞及对刺激的应激反应等实现的。Objective To Analyze the changes of gene expression profile of adrenal tissue in cervical spondylosis of vretebral artery type（ CSA）of model rats and exploring the mechanism of adrenal gland control on CSA. Methods Wistar rats were randomly divided into model group and normal group. The CSA model was established by composite modeling method. The total RNA was extracted from the adrenal gland after the model was successfully obtained. The gene expression profile was detected by whole gene chip,Gene Ontology（ GO）,Kyoto Encyclopedia of Genes and Genomes（ KEGG） signal pathway analysis. Results Compared with the normal group,the difference gene expression profile analysis showed that the total number of differential genes was 367（ ︳fold change︱〉 2,P〈0. 05）,among which 236 were up-regulated and 131 were down-regulated. The enrichment of gene GO functions a total of2843,involving the regulation of cell cycle,the regulation of stress stimulation,the regulation of fibroblast proliferation,the regulation of endothelial cell proliferation,etc. Participating in the regulation of signaling pathways about 135,including mitogen-activated protein kinase（ MAPK） signal transduction pathway,phosphatidylinositol 3 kinase/proteinkinase B（ PI3K/Akt） signal path,Hedgehog signal path,Wingless and Int（ Wnt） signal pathway and so on. Conclusion The regulation of CSA on adrenal gland is mainly to regulate the proliferation and migration of vascular endothelial cells and vascular smooth muscle cells,adhesion molecules,fibroblasts,and the stress response to stimulation.

关 键 词：基因芯片 肾上腺 椎动脉型颈椎病 基因表达谱 

分 类 号：R97[医药卫生—药品]