姜黄素对乳腺癌细胞的增殖与迁移及侵袭的影响及其作用机制  

Effect of curcumin on the viavility migration and invasion of breast cancer cells and its mechanism

作　　者：王巧琳[1] 何娜娜[1] 陆明[1] 王宁[1] 陈音[1] 

机构地区：[1]新疆医科大学附属中医医院肿瘤一科,乌鲁木齐830000

出　　处：《中国临床药理学杂志》2017年第18期1793-1796,共4页The Chinese Journal of Clinical Pharmacology

基　　金：新疆维吾尔自治区自然科学基金资助项目(2016DOIC144)

摘　　要：目的探讨姜黄素对乳腺癌细胞增殖、迁移及侵袭能力的影响及其作用机制。方法体外培养乳腺癌细胞MDA-MB-231,对照组分别给予二甲基亚砜(DMSO)10μL,处理48 h,低、中、高3个剂量实验组分别给予5,10,20μg·m L^(-1)姜黄素,处理48 h。用四甲基偶氮唑盐(MTT)法检测乳腺癌细胞增殖能力的变化,用TUNEL法检测乳腺癌细胞凋亡的程度;用划痕实验及Transwell侵袭实验分别检测乳腺癌细胞的迁移及侵袭能力,用Western Blot法检测相关蛋白表达水平。结果处理48 h后,对照组和中、高2个剂量实验组的细胞增殖能力分别为100.00%,67.63%和49.17%,细胞凋亡率分别为0.38%,6.44%和24.22%,细胞迁移能力分别为100.00%,55.33%和40.67%,侵袭细胞数为(104±8),(37±7),(25±9)个,磷酸化P38蛋白表达量分别为100.00%,57.63%和42.51%,对照组和中、高2个剂量实验组比较差异均有统计学意义(均P<0.05)。结论姜黄素能够降低P38磷酸化水平,从而抑制乳腺癌细胞的增殖、迁移及侵袭能力,抑制其凋亡,其机制有待进一步研究。Objective To explore the effects and the mechanisms of curcumin on in vitro viability,migration and invasion of human breast cancer cell line. Methods The test group（ the concentrations of curcumin were 5,10,20 μg·m L^（-1）,respectively） and control group were set in experiment. The inhibition effect of curcumin on the viability of MDA-MB-231 was determined with methyl thiazolyl tetrazolium（ MTT）. The apoptosis rate was determined with TUNEL assay. Themigration rate was determined with wounding assay. The invasion number of MDA-MB-231 was determined with tranwell assay. The expression of phosphor-P38 was determined with Western blot. Results Curcumin suppressed MDA-MB-231 cell viability in a concentration-dependent manner when compared to the control. In high-dose group,cell viability after 48 h of curcumin treatment was decreased 49. 17% compared to control group; In medium-dose group,cell viability after 48 h of treatment was decreased 67. 63%. In medium-dose group,the apoptosis rate after 48 h of curcumin treatment was increased 6. 44% compared to control group（ 0. 38%）; the apoptosis rate 48 h of curcumin treatment was increased24. 22% in high-dose group. In medium-dose group,the migration rateafter 48 h of curcumin treatment was decreased 55. 33% compared to control group; the migration rate 48 h of curcumin treatment was decreased 40. 67% in high-dose group. In medium-dose group,the invasion number after 48 h of curcumin treatment was decreased（ 37 ± 7） cell,compared to control group（ 104 ± 8） cell; the invasion number 48 h of curcumin treatment was decreased（ 25 ± 9） cell in high-dose group. In medium-dose group,the expression of phosphor-P38 after 48 h of curcumin treatment was decreased 57. 63% compared to control group; the expression of phosphor-P38 was decreased 42. 51% in high-dose group. Conclusion Curcumin suppresses human liver cancer cell MDA-MB-231 viability,migration and invasion by suppressing the expression of phosphorylation P38.

关 键 词：姜黄素 乳腺癌细胞 增殖 迁移 侵袭 

分 类 号：R28[医药卫生—中药学]