抗扑海因枇杷内生木霉菌株PY2与原始菌株Y2生物学特性差异分析  

Differential Analysis for Biological Characteristics of Endophytic Trichoderma PY2 Strain Resistance to Iprodione and Wild Y2 Strain From Loquat

作　　者：鲁海菊[1] 董慧婷 祁建珉 王传铭[1] 曾垄钢 

机构地区：[1]红河学院生命科学与技术学院,云南蒙自661199 [2]屏边县茶果站,云南屏边661299 [3]屏边县植保植检站,云南屏边661299

出　　处：《北方园艺》2017年第18期1-6,共6页Northern Horticulture

基　　金：红河学院应用型科学研究重点资助项目(XJY15Z06);国家自然科学基金资助项目(31660147);云南省应用基础研究计划资助项目(2016FB066)

摘　　要：以抗扑海因枇杷内生木霉PY2菌株和原始菌株Y2为研究对象,采用菌丝生长速率及血球计数法,研究了不同培养基、碳氮源、pH、温度和光照对其菌丝生长及产孢的影响,以期探索二者生物学特性差异。结果表明:2个菌株最大的差异在于产孢性状不同,抗扑海因菌株PY2产孢能力明显强于原始菌株Y2。PY2菌株菌丝生长最佳条件为PDA、CDA或BDA培养基,葡萄糖,酵母膏,pH 5、6、8、9、10,温度为15~28℃,全黑暗或全光照;其产孢最佳条件为CDA培养基,D-果糖,牛肉膏,pH 3,25℃,全光照。In order to make clear difference of biological characteristics of TrichodermaPY2 resistant to iprodione and wild Y2 strain from loquat,the effects of medium,carbon and nitrogen source,pH,temperature and illumination on mycelial growth and sporulation quantity for PY2 and Y2strains were studied by method of mycelial growth rate and cell count technique.The results showed that characteristic of generating spores was significantly different between PY2 and Y2.PY2 was more powerful than Y2 in ability of generating spores.The proper conditions of mycelial growth were mediums of PDA,CDA or BDA,glucose,yeast extract and pH 5,6,8,9,10,15-28 ℃,all dark or all light.The proper conditions of generating spores were medium of CDA,D-fructose,beef extract,pH 3,25 ℃ and all light.

关 键 词：木霉 抗扑海因 生物学特性 

分 类 号：S667.3[农业科学—果树学]