一株白桩菇属野生食用菌菌种分离鉴定与培养  

Isolation,Identification and Training for a Wild Fungus of Genus Leucopaxillus

作　　者：刘晓婷[1] 孙国琴[2] 李亚娇[2] 王淑妍[1] 郭九峰[1] 

机构地区：[1]内蒙古大学物理科学与技术学院离子束生物工程自治区重点实验室,内蒙古呼和浩特010021 [2]内蒙古农牧业科学院,内蒙古呼和浩特010031

出　　处：《北方园艺》2017年第18期161-166,共6页Northern Horticulture

基　　金：国家自然科学基金资助项目(51267014)

摘　　要：以内蒙古锡林郭勒草原一株野生蘑菇为试材,采用形态特征和生态习性鉴定及分子鉴定的方法,对该蘑菇的菌丝体基因组DNA进行18S和ITS片段的扩增、测序,测序结果经Blast比对,从而对该蘑菇进行分离鉴定。结果表明:该野生真菌属于白桩菇属Leucopaxillus。另外,通过优化的MS平板培养基避光培养,在MS平板培养基上有原基形成,为日后对该菌种的驯化与栽培提供材料,为其开发与利用奠定基础。其ITS序列已上传GenBank,登录号为KY173356。A wild mushroom from Inner Mongolia′s Xilingol grassland was identified by methods of morphological characteristics and ecological habits of identification and molecular identification.Genomic DNA was extracted from mycelium then used as template for the 18 Sand ITS gene PCR amplification and sequencing.The 18 Sand ITS gene sequences were submitted to NCBI and aligned by Blast.The results showed that the fungi belonged to the genus Leucopaxillus.In addition,it had been found experimentally that the mycelium could be formed primordium in MS plate culture medium in dark.The ITS sequence was uploaded into NCBI,and GenBank accession number was KY173356.

关 键 词：野生真菌 形态特征 ITS序列 分子鉴定方法 原基 

分 类 号：S646.103.6[农业科学—蔬菜学]