单唾液酸四己糖神经节苷脂在模拟人体胃肠环境中稳定性研究  

Stability monosialotetrahexosylganglioside in simulated conditions of Human stomach and intestines

作　　者：邓龙[1,2] 熊英 王小青 邹晓青 

机构地区：[1]湘潭大学化学学院,湖南湘潭411105 [2]湖南省药物安全评价研究中心,新药药效与安全性评价湖南省重点实验室,长沙410331

出　　处：《中南药学》2017年第8期1072-1074,共3页Central South Pharmacy

基　　金：湖南省新药药效与安全性评价重点实验室开放性课题(No.201702)

摘　　要：目的考察单唾液酸四己糖神经节苷脂(GM1)在模拟人工胃肠环境中的稳定性。方法采用HPLC分别测定GM1在稀盐酸溶液、人工胃液以及在pH=6.8的磷酸盐缓冲液、人工肠液中8 h内的稳定性。结果 GM1(浓度100 mg·L^(-1)),在稀盐酸溶液及pH=6.8的磷酸盐缓冲液中连续孵育8 h测定的百分含量分别为(98.70±9.32)%、(97.65±4.76)%;在人工胃液及人工肠液中其连续孵育8 h测定的百分含量分别为(97.42±6.18)%、(100.91±5.50)%。结论 GM1(浓度100 mg·L^(-1))在模拟人工胃肠环境中以37℃恒温孵育8 h后稳定性良好。Objective To determine the stability of monosialotetrahexosylgang-lioside（GM1） in simulated conditions of human stomach and intestines. Methods The stability of GM1 in different conditions for 8 hours was determined by HPLC, namely in dilute hydrochloric acid solution, artificial gastric juice, buffer phosphate（pH = 6.8） and artificial intestinal juice. Results After incubation for 8 h in simulated conditions of human stomach and intestines at 37 ℃, the content of GM1（100 mg·L^（-1）） in dilute hydrochloric acid solution and buffer phosphate（pH = 6.8） was（98.70±9.32）% and（97.65±4.76）%, respectively. The content of GM1 in artificial gastric juice and artificial intestinal juice was（97.42±6.18）% and（100.91±5.50）%, respectively. Conclusion GM1（100 mg·L^（-1）） is stable in simulated conditions of human stomach and intestines after incubation for 8 h.

关 键 词：单唾液酸四己糖神经节苷脂 模拟人体胃肠环境 稳定性 

分 类 号：R554.9[医药卫生—血液循环系统疾病]