破血化瘀填精补髓方对糖尿病大鼠脑出血急性期血肿周围组织修复的影响  

Effect of Poxue Huayu Tianjing Busui Prescription on Perihematomal Tissue Repair of Diabetic Rats in Acute Cerebral Hemorrhage

作　　者：徐艳[1] 王剑[1] 任红[1] XU Yan WANG Jian REN Hong(Qingdao Municipal Hospital, Qingdao Shandong China 266011)

机构地区：[1]青岛市市立医院,山东青岛266011

出　　处：《中医学报》2017年第9期1620-1624,共5页Acta Chinese Medicine

基　　金：山东省中医药科技发展计划项目(2013-310)

摘　　要：目的:观察破血化瘀填精补髓方对糖尿病大鼠脑出血急性期血肿周围组织修复的影响。方法:选取健康Wistar大鼠120只为研究对象,建立糖尿病大鼠脑出血模型后,随机分为模型组(生理盐水灌胃)、阳性对照组(醒脑健神胶囊灌胃)及研究组(破血化瘀填精补髓方灌胃),每组均40只。比较3组大鼠脑出血面积、神经功能缺损程度、脑水肿含水量、神经生长因子(nerve growth factor,NGF)、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)蛋白表达情况。结果:造模后第3天、第7天,研究组血肿面积比值分别为0.06±0.01、0.01±0.01,模型组血肿面积比值分别为0.08±0.01、0.05±0.01,两组比较,差异均有统计学意义(P<0.05)。造模后第3天、第5天、第7天,研究组Longa评分分别为(2.03±0.32)分、(0.92±0.47)分、(0.15±0.09)分,模型组Longa评分分别为(3.12±0.42)分、(2.22±0.38)分、(0.52±0.21)分,两组比较,差异均有统计学意义(P<0.05)。造模后第3天、第7天,研究组脑水肿含量分别为(71.25±8.12)%、(66.58±6.24)%,模型组脑水肿含量(88.56±10.23)%、(78.36±7.26)%,两组比较,差异均有统计学意义(P<0.05)。造模后第7天,研究组NGF阳性细胞数为(24.63±5.21)个,模型组为(10.58±3.66)个,两组比较,差异有统计学意义(P<0.05)。造模后第1天、第3天、第5天、第7天,研究组BDNF阳性细胞数分别为(22.47±3.16)个、(32.47±3.55)个、(40.57±4.21)个、(50.23±5.77)个,模型组BDNF阳性细胞数分别为(14.63±3.21)个、(14.89±3.56)个、(14.82±3.75)个、(15.02±3.45)个,两组比较,差异均有统计学意义(P<0.05)。结论:破血化瘀填精补髓方可显著促进糖尿病大鼠脑急性出血期血肿周围组织的修复,改善大鼠神经功能缺损情况,其作用机制可能与上调NGF、BDNF蛋白表达有关。Objective：To investigate the effect of Poxue Huayu Tianjing Busui Prescription on perihematomal tissue repair of diabetic rats in acute cerebral hemorrhage.Methods：120 healthy Wistar rats were selected as research object.After establishing diabetic rat models with cerebral hemorrhage,they were randomly divided into model group（gavaged with saline）,positive control group （gavaged with Xingnao Jianshen capsules）and research group（gavaged with Poxue Huayu Tianjing Busui Prescription）,with 40cases in each group.The cerebral hemorrhage area,neurological function deficits scale（NFDS）,nerve growth factor（NGF）,brain-derived neurotrophic factor（BDNF）protein expression of the three groups were compared.Results：Three and seven days after modeling,the hematoma area ratio of the study group was（0.06±0.01）and（0.01±0.01）respectively.The ratio of hematoma area in the model group was（0.08±0.01）and（0.05±0.01）respectively.There were significant differences between the two groups（P〈0.05）.On the 3rd day,the 5th day and the 7th day after modeling,the Longa scores of the study group were（2.03±0.32）,（0.92±0.47）and（0.15±0.09）,and the Longa scores of the model group were（3.12±0.42）,（0.22±0.38）and（0.52±0.21）.The differences were statistically significant（P〈0.05）.On the third day and the seventh day after modeling,the brain edema contents of the study group were（71.5±8.12）%and（66.58±6.24）%,and that in the model group were（88.56±10.23）%and（78.36±7.26）%respectively.The differences between the two groups were statistically significant（P〈0.05）.On the 7th day after modeling,the number of NGF positive cells in the study group was（24.63±5.21）and that in the model group was（10.58±3.66）.The difference was statistically significant（P〈0.05）.On the first day,the third day,the fifth day and the seventh day after modeling,the number of BDNF positive cells in the study group were（22.47±3.16）,（32.47±3.55）,（40.57�

关 键 词：破血化瘀填精补髓方 糖尿病 脑出血 组织修复 神经生长因子 脑源性神经营养因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]