壮医方“白花丹消蛊汤”对食蟹猴肝纤维化的干预作用研究  

Study on Effect of Zhuang Medicine "Baihuadan Xiaogu Tang"(白花丹消蛊汤) on Experimental Liver Fibrosis in Cynomolgus Macaque

作　　者：陈少锋 赵湘培 苏华[2] 阳明 覃子瑞 黄卫宣 余胜民 钟鸣[2] CHEN Shao-feng ZHAO Xiang-pei SU Hua YANG Ming QIN Zi-rui HUANG Wei-xuan YU Sheng-ming ZHONG Min(Guangxi Institute of Nationality Medicine, Nanning Guangxi 530001, China Guangxi Institute of Chinese Medicine ＆ Pharmaceutical Science, Nanning Guangxi 530012, China Changcun Biological Tech. Co., Ltd. of Fangchenggang City, Fangchenggang Guangxi 538038, China)

机构地区：[1]广西壮族自治区民族医药研究院,广西南宁530001 [2]广西中医药研究院,广西南宁530012 [3]广西防城港常春生物技术开发有限公司,广西防城港538038

出　　处：《中医药导报》2017年第17期21-24,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西科学研究与技术开发计划课题(No.桂科能14123006-6)

摘　　要：目的:研究壮医方白花丹消蛊汤对食蟹猴肝纤维化的干预作用。方法:将12只食蟹猴随机分为4组,除空白对照组外,其余各组每周2次皮下注射0.5 mL/kg 40%四氯化碳(CCl4)橄榄油剂造模,同时辅以高脂饲料喂养,建立食蟹猴肝纤维化模型。造模6个月后,各组相应给药,白花丹消蛊汤低、高剂量组分别给予1.0、4.0 g(生药)/(kg·d)灌胃,空白组和模型组予等体积的生理盐水。给药8周后,静脉采集食蟹猴血液样本,检测各组食蟹猴血清中丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总胆红素(T-BIL),酶联免疫法(ELISA)检测食蟹猴血清中血小板衍生生长因子(PDGF)和白介素1β(1L-β)水平,苏木素伊红(HE)染色和Masson染色观察肝纤维化程度。结果:白花丹消蛊汤低、高剂量组血清ALT、AST、ALP、T-BIL、PDGF水平均低于模型组;与模型组比较,白花丹消蛊汤低、高剂量组基质金属蛋白酶、免疫组化、肝组织纤维化程度明显改善。结论:壮医方白花丹消蛊汤具有抗肝纤维化作用。Objective： To observe the effect of Zhuang medicine prescription of Baihuadan Xiaogu Tang in hepatofibrosis on cynomolgus macaque. Methods： The 12 Cynomolgus macaques were randomly divided into 4 groups. All cynomolgus macaques, except the animal of normal group, were induced by injection of 40% carbon tetrachloride hypodermically twice a week at dose of 0.5 mL/kg, and at the same time animal were given rich fat and drug diet. Six months after the modeling, Baihuadan Xiaogu Tang low dose group and high dose group were givenl.0 and 4.0 g/（kg·d） by garage respectively; blank group and model group were given equal volume of saline. After 8 weeks all cynomolgus macaques were sacrificed. The enzyme＇s content of ALT, AST, ALP, T-BIL and factor＇s leve of PDGF, 1L-β from blood were determined. The hepatofibrosis were observed through the staining of HE and Masson. Results： Baihuadan Xiaogu Tang low dose group and high dose group showed lower content of ALT, AST, ALP, T-BIL and PDGF from blood than model group. Compared with the model group, matrix metalloproteinase, immunohistochemistry, liver fibrosis significantly improved in Baihuadan Xiaogu Tang low dose group and high dose group. Conclusions： The prescription of Baihuadan Xiaogu Tang is capable of anti- hepatofibrosis action.

关 键 词：肝纤维化 白花丹消蛊汤 PDGF 1L—β 食蟹猴 

分 类 号：R285.5[医药卫生—中药学]