HPLC法同时测定强肾益精颗粒中四种成分含量研究  

Study on Simultaneous Determination four Components of Qiangshen Yijing Granules(强肾益精颗粒) by HPLC

作　　者：欧阳波[1] 肖作奇 潘涛[1] 黄懿[1] 田霞[1] OUYANG Bo XIAO Zuo-qi PAN Tao HUANG Yi TIAN Xia(Hu＇nan Provincial Maternal and Child Health Care Hospital, Changsha Hu＇nan 410008, China)

机构地区：[1]湖南省妇幼保健院,湖南长沙410008

出　　处：《中医药导报》2017年第17期53-56,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省中医药管理局科研项目(201627);长沙市科技局科技项目(K15ZD008-33)

摘　　要：目的:建立同时测定强肾益精颗粒中王不留行黄酮苷、松果菊苷、金丝桃苷及毛蕊花糖苷等4种成分含量的方法。方法:采用HPLC法,色谱柱为Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm);流动相为0.1%磷酸溶液-乙腈,梯度洗脱,流速为1.0 mL/min;检测波长为330 nm;柱温为25℃;进样量为10μL;检测时间为90 min。结果:王不留行黄酮苷、松果菊苷、金丝桃苷和毛蕊花糖苷分别在11.6~116.6μg/mL(R^2=0.999,7)、15.1~151.4μg/mL(R2=0.999,4)、6.0~60.0μg/mL(R2=0.999,5)、3.3~33.0μg/mL(R^2=0.999,8)浓度范围内与其峰面积呈良好的线性关系;王不留行黄酮苷、松果菊苷、金丝桃苷及毛蕊花糖苷平均加样回收率分别为100.1%、102.0%、99.3%和98.1%,RSD%值分别为1.33%、1.09%、1.76%和1.37%。结论:该方法结果准确、重复性好,可用于强肾益精颗粒主要成分含量控制。Objective： To establish a method for the determination of Vaccarin, Echinacoside, Hyperoside and Verbascosidein Qiangshen Yijing Granules by high performance liquid chromatography （HPLC）.Methods： HPLC was used; the column was Agilent TC-C18 column （250 mm×4.6 mm,5 μm）; the gradient elution solvent system consisted of 0.1% H3PO4 water-acetonitrile solution at the flow rate of 1.0 mL/min; the detection wavelength was 330 nm; the column temperature was 25 ℃; the injection volume was 10 μL, and the detection time was 90min Results： Vaccarin, echinacoside, hyperoside and verbascoside showed a good linearity at 11.6--116.6μg/mL （R2=0.999,7）, 15.1-151.4 μg/mL （R2=0.999,4）, 6.0-60.0 μg/mL （R2=0.999,5） and 3.3-33.0 μg/mL （R2=0.999,8）, respectively. The average recoveries （RSD%） were 101.1% （1.33%）, 102.0% （1.09%）, 99.3% （1.76%） and 98.1% （1.37%）, respectively. Conclusion： The method is accurate, repeatability and suitable to be used for the quality control of the main components in Qiangshen Yijing Granules.

关 键 词：HPLC法 强肾益精颗粒 王不留行黄酮苷 松果菊苷 金丝桃苷 毛蕊花糖苷 

分 类 号：R284.1[医药卫生—中药学]