生长分化因子15对骨髓间充质干细胞增殖及基因表达的影响  

Effect of the growth differentiation factor 15 on rat bone marrow-derived mesenchymal stem cell proliferation and gene expression

作　　者：戈旌[1] 李萌宇 华洪飞 王宁涛[1] 汪湧[1] 张志愿[1] 王绍义[1] GE Jing LI Mengyu HUA Hongfei WANG Ningtao WANG Yong ZHANG Zhiyuan WANG Shaoyi.(Department of Oral Surgery, Ninth People＇s Hospital, Shanghai Jiaotong University School of Medicine, Key Laboratory of Stomatology, Shanghai 200011, China)

机构地区：[1]上海交通大学医学院附属第九人民医院口外科、上海市口腔医学重点实验室,上海200011

出　　处：《口腔医学》2017年第9期778-784,共7页Stomatology

基　　金：国家自然科学基金(81271114)

摘　　要：目的探讨生长分化因子15(GDF15),对大鼠骨髓间充质干细胞(BMSCs)增殖的调控作用及其机制。方法分离、培养大鼠BMSCs,流式细胞术和免疫荧光染色鉴定BMSCs;CCK8检测不同浓度rh-GDF15对BMSCs增殖的影响,流式细胞周期检测不同浓度rh-GDF15对BMSCs细胞周期的影响;Realtime PCR检测不同浓度rh-GDF15对BMSCs中ALP、RunX2、COL-1、VEGF、b FGF和HIF-1α基因表达的影响。结果常氧条件下,GDF15可以在48 h内促进BMSCs的增殖,并上调VEGF的基因表达,且具有浓度相关性,50μg/L的rh-GDF15可以显著降低BMSCs的G1期,上调S期和G2期细胞比例,促增殖作用最显著。结论 GDF15可以在短时间内促进BMSCs的增殖和VEGF基因表达,其机制还需进一步探索。Objective To investigate the regulatoty effect of GDF15 on the proliferation and gene expression of BMSCs and its mechanism.Methods The BMSCs were isolated and cultured from rat long bones.The proliferation and cell cycle of BMSCs cultured with different concentrations of recombinant GDF15 proteins were tested by Cell Counting Kit-8 and flow cytometry respectively.The mRNA levels of ALP,RunX2,COL-1,VEGF,b FGF and HIF-1α in BMSCs under induction of GDF15 were detected by real time PCR.Results Under normoxia condition,GDF15 promoted proliferation,cell cycle progress and mRNA level of VEGF in BMSCs dose-dependently,and 50 μg/L was the optimum concentration.Conclusion GDF15 promotes proliferation and VEGF gene expression in BMSCs in short time,but its mechanism still needs further study.

关 键 词：生长分化因子15 骨髓干细胞 增殖 

分 类 号：R349.51[医药卫生—基础医学]