响应面法优化海参多肽脱色工艺  

Optimization of Decoloring Process of Sea Cucumber Hydrolysates by Response Surface Methodology

作　　者：胡庆苹 裴栋 姜红梅[4] 祝潇[1,2] 魏鉴腾 王宁丽 邸多隆[1,2] 

机构地区：[1]青岛市资源化学与新材料研究中心,山东青岛266100 [2]中国科学院兰州化学物理研究所中科院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000 [3]兰州大学公共卫生学院营养与食品卫生学研究所,甘肃兰州730000 [4]蓬莱市疾病预防控制中心,山东烟台265600

出　　处：《食品研究与开发》2017年第16期71-76,共6页Food Research and Development

基　　金：国家自然科学基金(21605149);中科院STS项目(KFJSW-STS-146);青岛市民生计划(15-9-2-97-nsh)

摘　　要：为除去海参多肽中有色物质以利于生物活性肽的分离纯化,经酶解法制备海参多肽酶解液,以脱色率和多肽回收率为评价指标,考察不同脱色剂对海参多肽酶解液的脱色效果,从中筛选出XX型粉末活性炭。在单因素试验的基础上,利用响应面法对脱色条件进行优化。最佳脱色条件为:活性炭用量0.9 g/100 mL,时间32 min,温度50℃,pH 2.2,在此条件下,海参多肽液的脱色率达到(85.39±2.25)%,多肽回收率达到(88.57±1.86)%,与理论值无显著差异。In order to separate and purify the bioactive peptide,the color material of sea cucumber hydrolysates should be removed. The sea cucumber hydrolysates were made by enzyme hydrolysis. Based on the decoloring rate and peptide retention rate detected,the different bleaching agent was investigated,and the XX powder activated carbon was chosen as the best. Based on the results of single factor experiments,the decoloring process was optimized by response surface methodology. The results showed that the optimal conditions were as follows：the amount of activated carbon 0.9 g/100 mL,time 32 min,temperature 50 ℃,pH 2.2. Under this optimal condition,the decoloring rate was（85.39 ± 2.25）%,the peptide retention rate was（88.57 ± 1.86）% which was not significant with the theoretical value.

关 键 词：海参多肽 活性炭 脱色 响应面法 

分 类 号：TQ936.16[化学工程]