有机铵盐阳离子对Fe取代Keggin型磷钨酸盐的结构、形貌及体外抗肿瘤活性的影响  

Effects of Organic Ammonium Cations on Structure,Morphology and in vitro Anti-tumor Activity of Fe(Ⅲ)-Substituted Keggin-Type Phosphotungstates

作　　者：薛莹雪 隋春红 王程 李文亮 白羽 XUE Yingxue SUI Chunhong WANG Cheng LI Wenliang BAI Yu(College of Pharmacy, Jilin Medical University, Jilin 132013, Jilin Province, China)

机构地区：[1]吉林医药学院药学院,吉林吉林132013

出　　处：《吉林大学学报（理学版）》2017年第5期1329-1335,共7页Journal of Jilin University:Science Edition

基　　金：吉林省教育厅科学技术研究项目(批准号:2015398);吉林省卫生计生青年科技骨干培养项目(批准号:2016Q055);吉林市科技计划项目(批准号:201464049)

摘　　要：以有机铵盐(十六烷基三甲基溴化铵(CTAB),四乙基溴化铵(TEAB),四甲基溴化铵(TMAB))作为阳离子,合成3种单Fe取代的Keggin型磷钨酸盐[CTA]_4PW_(11)Fe(H_2O)O_(39)(CTAFe),[TEA]_4PW_(11)Fe(H_2O)O_(39)(TEAFe)和[TMA]_4PW_(11)Fe(H_2O)O_(39)(TMAFe),并通过红外(IR)光谱、紫外-可见(UV-Vis)光谱、X射线衍射光谱(XRD)及扫描电子显微镜(SEM)等考察有机铵盐阳离子对其结构及颗粒形貌的影响,应用四甲基偶氮唑盐(MTT)比色法对比分析3种磷钨酸盐对人源结肠癌细胞HCT116和HT29及鼠源结肠癌细胞CT26的增殖抑制活性.结果表明:3种磷钨酸盐仍保持Keggin型结构,但引入不同的有机铵盐阳离子使其颗粒形貌不同,有机铵盐阳离子的碳链越长,得到的颗粒结晶度越差,CTAFe已不再呈现明显的晶体形貌;TEAFe和TMAFe对HCT116,HT29和CT26细胞无明显的增殖抑制作用,CTAFe对3种肿瘤细胞均有抑制作用,具有药物浓度依赖性,对CT26和HCT116细胞的抑制作用较强,IC_(50)值分别为0.038,0.08mg/mL;烷基链较长的有机铵盐阳离子,其磷钨酸盐具有更好的抗肿瘤活性.Three mono-Fe（Ⅲ）-substituted Keggin-type phosphotungstates with different organic ammonium cations were systhesized：[CTA]4PW（11）Fe（H2O）O（39）（CTAFe）,[TEA]4PW（11）Fe（H2O）O（39）（TEAFe）and [TMA]4PW（11）Fe（H2O）O（39）（TMAFe）（CTA=cetyl trimethyl ammonium bromide,TEA=tetraethyl ammonium bromide,TMA=tetramethyl ammonium bromide）.The structure and morphology of three phosphotungstates were characterized by infrared spectroscopy（IR）,UV-visible spectroscopy（UV-Vis）,X-ray diffraction（XRD）and scanning electron microscopy（SEM）.Theinhibitory effect of three phosphotungstates on the growth of human colorectal carcinoma HCT116,HT29 cells and rat colorectal carcinoma CT26 cells was studied by MTT assay.The results show that the phosphotungstates with different organic ammonium cations still keep Keggin-type structure.But the cations affect the morphology.Longer carbon chain of organic ammonium cations makes crystallinity decrease,no clear crystal morphology of CTAFe.TEAFe and TMAFe have no inhibition on HCT116,HT29 and CT26cell proliferation,but CTAFe can inhibit these three tumor cells proliferation, showing dose-dependent. The IC50 value for CT26 and HCT116 cell was 0.038,0.08mg/mL,respectively.So longer carbon chain of organic ammonium cation makes Keggintype phosphotungstates inhibit tumor cell growth stronger.

关 键 词：KEGGIN型 磷钨酸盐 抗肿瘤 有机铵盐 

分 类 号：O614[理学—无机化学]