萝卜种质资源对芜菁花叶病毒的抗性鉴定与评价  

Identification and Evaluation for The Resistance of Radish(Raphanus sativus L.) Germplasm Resources to Turnip Mosaic Virus

作　　者：杨好慧 邱杨[1] 宋江萍[1] 张晓辉[1] 王鸿娜 王超楠 王海平[1] 李锡香[1] YANG Hao-hui QIU Yang SONG Jiang-ping ZHANG Xiao-hui WANG Hong-na WANG Chao-nan WANG Hai-ping LI Xi-xiang(Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Beijing Research Station of Vegetable Crop Gene Resource and Germplasm Enhancement ,Ministry of Agriculture ,Beijing 100081)

机构地区：[1]中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点试验室,北京100081

出　　处：《植物遗传资源学报》2017年第5期810-818,共9页Journal of Plant Genetic Resources

基　　金："十三五"国家重大研发项目(2016YFD0100204-02);"十二五"国家科技支撑项目(2013BAD01B04-1);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)

摘　　要：病毒病是危害萝卜作物的主要病害之一,芜菁花叶病毒(TuMV)作为萝卜病毒病的主要毒源,给萝卜生产造成严重经济损失。由于缺乏精准鉴定,萝卜育种中缺乏稳定可靠的抗TuMV材料。本研究就国家蔬菜种质资源中期库提供的来自中国25个省份125个县市和其他3个国家的150份代表性萝卜种质资源对TuMV的抗性进行了苗期鉴定和ELISA检测,筛选出23份抗病材料,其中1份表现免疫。这些材料对萝卜抗病毒病基因定位和新品种培育具有重要意义。Virus disease is one of the major diseases of radish（ Raphanus sativus L.）. Turnip mosaic virus（ TuMV） which is the most important causative virus causes great economical loss in radish. The virus-resistant materials are less in radish due to lacking of precision identification. In this study,the resistance to TuMV was identified among 150 accessions of representative radish germplasm,coming from 125 counties of 25 provinces in China and 3 countries outside of China. As a result,one immune material and twenty-two resistant materials were screened out by combing phenotyping and ELISA detection. These materials would be useful for resistant gene mapping and new radish variety development in the future.

关 键 词：萝卜种质 芜菁花叶病毒 抗性鉴定 ELISA 

分 类 号：S436.31[农业科学—农业昆虫与害虫防治]