脑室内注射orexin-A和orexin-B对下丘脑组胺释放的影响  

Effect of microinjection of orexin-A and orexin-B into cerebral ventricle on the release of histamine in hypothalamus

作　　者：李龙[1] 王志华[2] 张丽娜[1] 董海龙[1] LI Long WANG Zhihua ZHANG Lina DONG Hailong(Department of Anesthesiology, Xijing Hospital, the Fourth Military Medical University, Xi ＇ an 710032, China)

机构地区：[1]第四军医大学附属西京医院麻醉科,西安市710032 [2]宁夏医科大学总医院麻醉科

出　　处：《临床麻醉学杂志》2017年第9期890-893,共4页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金(81371510);国家自然科学青年基金(81401138)

摘　　要：目的观察脑室内微注射orexin-A和orexin-B对下丘脑组胺释放的影响,为orexins促麻醉觉醒的机制提供新的解释。方法成年雄性SD大鼠随机分为三组:生理盐水组、orexin-A组和orexin-B组。在立体定位仪下将微透析管置入下丘脑结节乳头体核区。实验分为两部分:先收集脑室内分别注射orexin-A、orexin-B 1nmol和5nmol后1、2h和3h的微透析液(n=5),采用高效液相色谱法(HPLC)检测微透析液中组胺含量的时程变化。1周后,各组大鼠分别注入不同剂量的orexin-A和orexin-B(10、15nmol和20nmol,n=5),收集微注射后1h的透析液,检测组胺释放的剂量效应。在立体定位仪下结节乳头体核置入微注射管,1周后给予1.4%异氟醚(1 MAC)麻醉30min,各组大鼠分别微注射生理盐水、orexin-A和orexin-B 0.3μl(n=6),记录翻正反射恢复时间。结果与生理盐水组比较,脑室内微注射orexin-A 1nmol 2h后下丘脑组胺含量明显增加,但同样剂量的orexin-B无明显作用,注射orexin-A和orexin-B 5nmol后2、3h组胺含量均明显增加(P<0.01)。脑室内微注射10nmol、15nmol和20nmol的orexin-A和orexin-B后1h组胺含量明显高于生理盐水,且20nmol含量最高(P<0.05)。结节乳头体核微注射orexin-A后翻正反射恢复时间明显短于生理盐水(P<0.01),微注射orexin-B后无明显作用。结论脑室内微注射orexin-A和orexin-B均可促进下丘脑组胺递质的释放,但orexin-A的作用更强。结节乳头体核微注射orexin-A促进异氟醚麻醉的觉醒。Objective To provide new evidences for understanding the mechanisms of promotive role of orexins in anesthetic emergence and the effect of microinjection of orexin-A/orexinB into cerebral ventricle on the release of histamine.Methods Male SD rats were randomly divided into saline（control）,orexin-A and orexin-B groups.The microdialysis probe was inserted into hypothalamus under stereotaxic apparatus.The perfused fluid from the area of hypothalamic tuberomammillary nucleus was collected using in vivo microdialysis at 1h,2hand 3hafter 1nmol or 5nmol orexin-A or orexin-B microinjection into the cerebral ventricle（n=5each）.The concentrations of histamine at each time point in dialysates of perfused fluid were detected by high performance liquid chromatography（HPLC）to analyze its dynamic changes.After one week,each group was microinjected with 10 nmol,15nmol and 20 nmol orexin-A and orexin-B（n=5）into the cerebral ventricle respectively,dialysates was collect and histamine was detected at 1hto analyze its dosage response.After one week,each group was microinjected 0.3μl saline orexin-A and orexin-B（n=6）into the tuberomammillary nucleus.Results Compared with the control group,microinjection of 1nmol orexinA significantly increased histamine release at 1h,but the same dose of orexin-B had no such effect,5nmol of orexin-A or orexin-B injections significantly facilitated histamine release at 2hand 3h（P〈0.01）.Microinjection of 10 nmol,15nmol and 20 nmol orexin-A and orexin-B into ventricle caused an significant increase of histamine release at 1h while the effect was the strongest in 20nmol（P〈0.05）.Compared with the control group,microinjection of orexin-A significantly decreased time of the righting reflex（P〈0.01）,but the same dose of orexin-B had no such effect.Conclusion Microinjection of both orexin-A or orexin-B into cerebral ventricle could promote the release of histamine,while the effect of orexin-A was stronger.Microinjection orexin-A into tuberomammillary nucleus significant f

关 键 词：OREXIN-A Orexin-B 组胺 

分 类 号：R614[医药卫生—麻醉学]