棉铃虫对茚虫威的抗性机理：PBO,DEF和DEM的增效作用及解毒酶活性  

Mechanisms of resistance to indoxacarb in Helicoverpa armigera (Lepidoptera: Noctuidae) : the synergistic effects of PBO,DEF and DEM and the activities of detoxification enzymes

作　　者：王芹芹[1] 崔丽[1] 王奇渊 杨宏宇 芮昌辉[1] WANG Qin-Qin CUI Li WANG Qi-Yuan YANG Hong-Yu RUI Chang-Hui(Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China Jining Normal University, Wulanchabu, Inner Mongolia 012000, China)

机构地区：[1]中国农业科学院植物保护研究所,农业部作物有害生物综合治理重点实验室,北京100193 [2]集宁师范学院,内蒙古乌兰察布012000

出　　处：《昆虫学报》2017年第8期912-919,共8页Acta Entomologica Sinica

基　　金：国家重点研发计划(2016YFD0200500);公益性行业(农业)科研专项(201203038)

摘　　要：【目的】本研究旨在明确不同棉铃虫Helicoverpa armigera种群对茚虫威的抗性水平及抗性机理,以科学有效防治这一害虫,避免其对茚虫威的抗性快速发展。【方法】采用浸叶法测定了棉铃虫不同种群,即相对敏感种群(CP)、汰选种群(TP)、沂水种群(YP)(采自山东沂水)和邯郸种群(HP)(采自河北邯郸)3龄幼虫对茚虫威抗性水平及增效醚(PBO)、脱叶磷(DEF)、顺丁烯二酸二乙酯(DEM)对茚虫威的增效作用;并测定了不同种群棉铃虫3龄幼虫体内多功能氧化酶(MFO)、羧酸酯酶(CarE)和谷胱甘肽-S-转移酶(GST)3种解毒酶及乙酰胆碱酯酶(ACh E)活性。【结果】CP种群对茚虫威敏感,TP,YP及HP种群对茚虫威的抗性倍数(resistence ratio,RR)分别为4.36,8.06和15.34倍,分别表现为敏感性降低、低水平抗性和中等水平抗性。在TP种群中,当棉铃虫3龄幼虫取食3种增效剂处理的叶片时,在0,6和12 h处理时间内增效作用随时间而升高,且PBO和DEF对茚虫威的增效作用优于DEM。增效剂PBO,DEF和DEM饲喂棉铃虫3龄幼虫12 h后对HP种群的增效倍数分别为3.86,2.52和4.57倍,对CP种群增效作用分别为1.11,0.52和0.91倍。酶活力对比研究发现,HP种群的棉铃虫MFO,CarE和GST活性显著高于CP种群和TP种群,YP种群的棉铃虫MFO和CarE活性显著高于CP种群,CarE活性显著低于HP种群,但是ACh E活性在棉铃虫种群间差异不显著。【结论】结果提示,当达到中等抗性水平时,MFO,CarE和GST活性显著升高与棉铃虫对茚虫威的抗性有关,而代谢抑制剂PBO,DEF和DEM对茚虫威有明显的增效作用。【Aim 】 This study aims to clarify the levels and mechanisms of indoxacarb resistance in different populations of the cotton bollworm,Helicoverpa armigera,so as to scientifically control this insect pest and to effectively avoid the rapid development of resistance to indoxacarb. 【Methods】The resistance levels and the synergistic effects of PBO,DEF and DEM on indoxacarb in the 3rd instar larvae of different populations of H. armigera,including the susceptible population（ CP）,the indoxacarbselected population（ TP）,Yishui population（ YP） from Yishui,Shandong and Handan population（ HP） from Handan, Hebei, were determined with leaf-dipping method. The activities of three detoxification enzymes [mixed function oxidase（ MFO）,carboxylesterase（ CarE） and glutathione Stransferase（ GST） ]and acetylcholinesterase（ Ach E） in these populations were assayed. 【Results】The CP population of H. armigera was still susceptible to indoxacarb,while the TP,YP and HP populations exhibited decreased sensitivity,low-level resistance and moderate-level resistance to indoxacarb,with the resistance ratios of 4. 36-,8. 06-and 15. 34-fold,respectively. In the TP population,the toxicity of indoxacarb to the 3rd instar larvae of H. armigera increased when they were fed on the leaves treated by three synergists for 0,6 and 12 h. The synergistic effects of PBO and DEF to indoxacarb in the 3rd instar larvae were greater than that of DEM. After the 3rd instar larvae of H. armigera were fed on the leaves treated by PBO,DEF and DEM for 12 h,the synergistic ratios were 3. 86-,2. 52-,and 4. 57-fold in the HP population,while 1. 11-,0. 52-and 1. 09-fold in the CP population,respectively. The activities of MFO,CarE and GST in the HP population were significantly higher than those in the CP and TP populations. The activities of MFO and CarE in the YP population were significantly higher than those in the CP population,while the CarE activity in the YP population was significantly lower than that in the HP populatio

关 键 词：棉铃虫 茚虫威 抗药性 代谢抑制剂 增效作用 解毒酶 

分 类 号：Q965.9[生物学—昆虫学]