复方中草药对白斑综合征病毒感染下凡纳滨对虾免疫活性的影响  

Effects of Compound Chinese Herbs on Immune Activities of Litopenaeus vannamei After Infected with White Spot Syndrome Virus

作　　者：陈辉辉[1] 涂晨凌 唐杨 黄永春[1,2] 

机构地区：[1]集美大学水产学院 [2]福建省海洋渔业资源与生态环境重点实验室,福建厦门361021

出　　处：《厦门大学学报（自然科学版）》2017年第5期686-692,共7页Journal of Xiamen University：Natural Science

基　　金：福建省科技厅产学研项目(2017N51012);厦门市海洋经济创新发展区域示范项目(13PZY001SF25)

摘　　要：为研究复方中草药(内含葛根、黄连、金银花、板蓝根、黄芪、甘草、柴胡、当归、山楂、陈皮和茯苓)对白斑综合征病毒(WSSV)感染下凡纳滨对虾(Litopenaeus vannamei)免疫特性的影响,分别将0(对照组),0.4%,0.8%,1.2%,1.6%和2.0%(质量分数)复方中草药添加到基础饲料中,连续投喂凡纳滨对虾28d后,测定其生长指标及经WSSV感染0,12,24,48和96h后免疫相关酶(总超氧化物歧化酶(T-SOD)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、谷草转氨酶(GOT)、谷丙转氨酶(GPT))的活力和丙二醛(MDA)含量,并观察感染192h内的死亡情况.结果表明:经WSSV感染后,凡纳滨对虾的T-SOD、AKP、ACP、GOT在0~96h内的酶活力表现为先升后降,其中在24~48h内达到高峰期;整个实验过程中,0.8%和1.2%添加组对虾的5种免疫相关酶活力均处于较高水平且较其余组稳定,而MDA含量较低且在24~48h内与其余各组差异显著(p<0.05);感染192h时,0.8%添加组对虾的存活率最高((33.33±6.11)%),50%死亡时间最迟((150.50±48.88)h),对照组和0.4%添加组则全部死亡,并且50%死亡时间最早,死亡速率也最快.综上可见,复方中草药能提高对虾免疫性能和抗WSSV能力,其中0.8%添加量效果最佳.该结果可为复方中草药合理应用于凡纳滨对虾健康养殖提供科学依据.To study effects of compound Chinese herbs（including Radix acutellariae,R.puerariae,Coptidis rhizome,Lonicera japonica,R.isatidis,Astragalus radix,R.glycyrrhize,R.bupleuri,Angelica sinensis,Crataegus pinnalifida,Pericarpium Citrusa reticulatae and Poria cocos）on immunologic factors and white spot syndrome virus（WSSV）-resistant capacity of Litopenaeus vannamei,shrimps were fed 28 dwith basal diets supplemented with 0（control group）,0.4%,0.8%,1.2%,1.6%,and 2.0% of compound Chinese herbs,respectively.Immunologic factors and death status were determined and observed at 0,12,24,48 and 96h after infected with WSSV.The results showed that activities of superoxide dismutase（T-SOD）,alkaline phosphatase（AKP）,acid phosphatase（ACP）and glutamic-oxalacetic transaminase（GOT）firstly increased and then decreased during 0-96 h,which reached a peak during 24-48 hafter infected with WSSV.Meanwhile,the activities of the four immunologic enzymes above as well as glutamicpyruvic transaminase（GPT）of the 0.8% and 1.2% supplemental groups were kept at a high level and remained steady during the whole experiment.Moreover,the content of malonaldehyde（MDA）of the 0.8% and 1.2% supplemental groups was significantly lower than that in other groups in 24-48h（p〈0.05）.After 192 hinfected with WSSV,the highest survival rate（33.33%）and 50%death time（150.50±48.88h）appeared in the 0.8% supplemental group,while the earliest 50% death time and the fastest 100%death rate of shrimps appeared in the control group and 0.4% supplemental group.In conclusion,compound Chinese herbs can promote immunity performance and WSSV-resistant ability of L.vannamei,among which the 0.8% supplemental groups showed the best effect.These results will provide scientific basis for reasonable use of the compound Chinese herbs for the healthy culture of L.vannamei.

关 键 词：凡纳滨对虾 白斑综合征病毒 复方中草药 免疫反应 

分 类 号：S945.49[农业科学—水产养殖]