表面增强拉曼光谱法快速鉴别失活白色念珠菌  

Rapid identification of inactivated C.albicans by surface-enhanced Raman spectroscopy

作　　者：王科兵[1] 陈志红 郭步 陆国忠[1] 

机构地区：[1]解放军169医院药械科,湖南衡阳421001

出　　处：《药学实践杂志》2017年第5期422-426,共5页Journal of Pharmaceutical Practice

摘　　要：目的建立表面增强拉曼光谱(surface-enhanced Raman spectroscopy,SERS)法快速判别失活白色念珠菌。方法利用热、甲醛、杀菌药(两性霉素B)分别灭活白色念珠菌,然后利用SERS法对失活前后的白色念珠菌进行光谱考察比较。结果白色念珠菌灭活后的SERS光谱显示出相似的特征峰,且与失活前的光谱有明显差异。结论该实验方法快速、简便,实现了对失活白色念珠菌的快速检测,有望成为快速鉴别更多病原微生物失活状态的有利工具。Objective To develop a rapid method for the identification of inactivated C.albicans by surface-enhanced Raman spectroscopy （SERS）.Methods Live C.albicans cultures were exposed to heating, formaldehyde and fungicidal drug （amphotericin B）.The corresponding SERS spectra were acquired for the investigation and comparison.Results The spectra acquired with three different inactivation methods exhibited similar features of dead C.albicans, which showed significant difference from the spectra of the live culture.Conclusion This SERS method can identify the inactivated C.albicans rapidly.Hopefully it will provide a convenient tool for quick identification of other inactivated pathogenic microorganisms.

关 键 词：白色念珠菌 表面增强拉曼光谱法 失活 快速鉴别 

分 类 号：O657.37[理学—分析化学]