阿魏菇多糖佐剂增强犬卵透明带3(CZP3)DNA疫苗的免疫不育效果  

Pleurotus ferulae polysaccharide adjuvant enhances the immune infertility of CZP3DNA vaccine

作　　者：林君媛 王颖[1] 李金玉[1] 张富春[1] LIN Junyuan WANG Ying LI Jinyu ZHANG Fuchun(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, Chin)

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046

出　　处：《细胞与分子免疫学杂志》2017年第8期1009-1013,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：新疆维吾尔自治区科技支疆项目(201591134)

摘　　要：目的研究阿魏菇多糖(PFP)作为佐剂增强犬卵透明带3(CZP3)DNA疫苗(pc DNA3-CZP3)的免疫不育效果。方法将pc DNA3-CZP3单独或分别与铝佐剂、PFP联用,肌肉注射免疫小鼠3次,采用ELISA测定免疫小鼠抗血清特异性抗体滴度;流式细胞术检测树突状细胞(DC)的成熟和T淋巴细胞增殖;合笼计算窝仔数检测免疫不育效果。综合评价PFP和铝佐剂对CZP3 DNA疫苗免疫效果的影响。结果联用铝佐剂和PFP的pc DNA3-CZP3与单独免疫pc DNA3-CZP3的疫苗组相比,均能提高受免小鼠抗血清特异性抗体水平。PFP作为佐剂能够显著性的增强DC表面成熟标志物CD86+和MHCⅡ+的基因表达水平,促进T细胞增殖,并且显著降低小鼠的生育率和窝仔数。结论 PFP能够提高免疫小鼠的抗体水平,促进DC成熟和增强T淋巴细胞增殖,增强CZP3 DNA疫苗免疫小鼠的不育效果。Objective To investigate the effect of Pleurotus ferulae polysaccharide（ PFP） as an adjuvant on the infertility induced by canine zona pellucida 3（ CZP3） DNA vaccine（ pc DNA3-CZP3）. Methods Female mice were intramuscularly immunized with pc DNA3-CZP3 alone or co-immunized with pc DNA3-CZP3 and aluminum adjuvant or PFP for three times.CZP3-specific antibody titers of antiserum were determined by ELISA. The maturation of dendritic cells（ DCs） and the proliferation of T lymphocytes were detected by flow cytometry. The litter of the immunized mice were counted. Results Compared with pc DNA3-CZP3 alone, pc DNA3-CZP3 combined with aluminum or PFP significantly increased the CZP3-specific antibody titers. Moreover,PFP as an adjuvant significantly up-regulated the expressions of CD86 and MHCⅡon DCs,enhanced the proliferation of T cells,and decreased the fertility rate and mean litter. Conclusion PFP can enhance the infertility efficacy of CZP3 DNA vaccine through increasing the humoral and cellular immune responses,promoting DC maturation and T cell proliferation.

关 键 词：犬卵透明带蛋白3(CZP3) DNA疫苗 佐剂 阿魏菇多糖 免疫不育效果 

分 类 号：R285.5[医药卫生—中药学] R392.1[医药卫生—中医学]