合并骨侵蚀及间质性肺病的类风湿关节炎患者血清IL-25水平升高  

Elevated serum IL-25 levels in rheumatoid arthritis patients with bone erosion and interstitial lung disease

作　　者：鲁锦玥 达明莲 封昱辰 刘媛媛[2] 张思功[2] 沈海丽[2] LU Jinyue DA Minglian FENG Yuchen LIU Yuanyuan ZHANG Sigong SHEN Haili(Second Clinical Medical College, Lanzhou University, Lanzhou 730000 Department of Rheumatology, Lanzhou University Second Hospital, Lanzhou 730000, China)

机构地区：[1]兰州大学第二临床医学院,甘肃兰州730000 [2]兰州大学第二医院风湿免疫科,甘肃兰州730000

出　　处：《细胞与分子免疫学杂志》2017年第8期1118-1122,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81560267);兰州大学第二医院博士科研基金(ynbskyjj2015-1-14)

摘　　要：目的分析类风湿关节炎(RA)患者疾病发展进程中血清白细胞介素25(IL-25)和IL-17水平以及与骨侵蚀及合并间质性肺病(ILD)的关系。方法 ELISA检测117例RA患者和56例健康对照血清IL-25和IL-17水平;检测血沉(ESR)、C反应蛋白(CRP)、类风湿因子(RF)、抗环瓜氨酸(CCP)抗体水平;胸部高分辨率CT(HR-CT)诊断ILD;肌骨超声检查判断骨侵蚀情况;Sharp评分划分影像学等级;VAS评分和28个关节疾病活动度评分(DAS28)反映RA患者的疾病活动情况。比较各组之间IL-25和IL-17的水平差异并分析相关性。结果 RA组的IL-25和IL-17水平明显高于健康对照组,有骨侵蚀的RA患者IL-25水平显著高于无骨侵蚀的RA患者。有ILD的RA患者IL-25水平显著高于不伴有ILD的RA患者。IL-25水平与RF-IgG、RF-IgA和RF-IgM均呈正相关(r=0.285、0.314、0.380),同时,IL-17水平与RF-IgG和RF-IgM呈正相关(r=0.198、0.273),但二者与抗CCP抗体之间无相关性。结论合并骨侵蚀及间质性肺病的类风湿关节炎患者血清IL-25水平升高。Objective To detect the serum levels of IL-25 and IL-17 in rheumatoid arthritis（ RA） patients and investigate the potential relationship with bone erosion and concomitant interstitial lung disease（ ILD）. Methods The study enrolled a total of 117 RA patients and 56 healthy subjects as controls. The serum levels of IL-25 and IL-17 were determined by ELISA,and rheumatoid factor（ RF） was detected by turbidimetric immunoassay,anticyclic citrullinated peptide（ anti-CCP） antibody as well as erythrocyte sedimentation rate（ ESR） and C-reactive protein（ CRP） were also tested. ILD was identified on high-resolution computed tomography（ HR-CT）,the degree of bone erosion was inspected by musculoskeletal ultrasonography,and radiographic grade was graded by Sharp-van der Heijde Score（ SHS）. Disease activity in RA was scored with the DAS28 and visual analogue scale（ VAS）. Correlation analysis was used to evaluate the correlations of IL-25 and IL-17 in different groups. Results Compared with healthy control group,the serum levels of IL-25 and IL-17 increased significantly in the patients with RA. Compared with bone erosion negative group,the serum level of IL-25 was higher significantly in bone erosion group. The level of IL-25 was higher in the ILD group of RA patients than the non-ILD group. In addition,there were positive correlations between the serum level of IL-25 and RF-IgG（ r = 0. 285）,RF-IgA（ r = 0. 314）,RF-IgM（ r = 0. 380）.Meanwhile,the serum level of IL-17 had the positive correlations with RF-IgG（ r = 0. 198） and RF-IgM（ r = 0. 273）. Both of them had no correlations with anti-CCP antibody. Conclusion The serum level of IL-25 is raised in RA patients with bone erosion and ILD.

关 键 词：类风湿关节炎 细胞因子 骨侵蚀 间质性肺病 炎症 

分 类 号：R593.22[医药卫生—内科学] R392.12[医药卫生—临床医学]