C末端是植物Fes1蛋白的必需功能域  

C-termianl is Essential Domain of Plant Fes1

作　　者：刘霞霞[1] 杨颖[1] 付灿[1] 于洪斌[1] 刘箭[1] LIU Xiaxia YANG Ying FU Can YU Hongbin LIU Jian(College of Life Science, Shandong Normal University, Jinan 250014, China)

机构地区：[1]山东师范大学生命科学学院,山东济南250014

出　　处：《济南大学学报（自然科学版）》2017年第4期304-310,共7页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金项目(31270298)

摘　　要：为了研究植物Fes1蛋白C末端特有的约40个氨基酸序列的生物学功能,以拟南芥为实验材料,将C末端缺失的AtFes1A基因转化进atfes1a突变体中,研究AtFes1A蛋白C末端序列是否为功能必需的结构域。结果表明,C末端缺失的AtFes1A可以表达稳定的转录本和蛋白,但C末端缺失的AtFes1A无法修复atfes1a的热敏感性,并且转基因株系中的热激蛋白HSP70的表达水平也未恢复,因此植物Fes1的C末端是植物特有且功能必需的。To study the biological function of about 40 amino acid sequences specific to the C-terminal of the plant Fesl, taking Arabidopsis thaliana as the experimental material to introduce the C-terminal deletion of AtFeslA gene into the atfesla mutant to investigate whether the C-terminal sequence of AtFeslA protein was an indispensable functional domain. The results show that AtFeslA without C-terminal can express stable transcripts and proteins. But AtFeslA without C-terminal presents neither remedy in heat-sensitive defect of atfesla nor the recovery of cytosolic HSP70 level. These findings suggest that the C-terminal is plant-specific and essential for normally performing the function of Fesl in plants.

关 键 词：C末端结构域 热激蛋白 耐热性 

分 类 号：Q945[生物学—植物学]