南苜蓿总皂苷对2型糖尿病大鼠胰腺功能保护作用的研究  

Research of protective effect of TSMP on pancreatic function in type 2 diabetic rats

作　　者：程静[1] 吴涛[1] 宋红萍[1] 陈镜楼 黄徐英[1] 刘萍[1] 

机构地区：[1]武汉市普爱医院药学部,武汉430033

出　　处：《中药药理与临床》2017年第4期53-56,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：武汉市青年科技晨光计划资助项目(NO:2015071704011629);武汉市卫生计生委中医药类重点课题资助项目(NO:WZ15A01)

摘　　要：目的:观察南苜蓿总皂苷对2型糖尿病大鼠胰腺的保护作用。方法:建立2型糖尿病大鼠模型,除对照组外,将成模大鼠分为模型组、二甲双胍组(0.2 g/kg)、南苜蓿总皂苷组(2.8 g/kg、1.4 g/kg),分别治疗4周。末次给药后测定空腹血糖(FBG)、空腹胰岛素(FINS)、糖化血红蛋白(Hb A1c)、肝糖原,取全血测定血液流变性。取胰腺组织匀浆测定SOD、MDA、GSH-Px、IL-1β、TNF-α。提取胰腺组织总蛋白,采用蛋白免疫印迹法检测胰岛内质网应激蛋白(CHOP、JNK)和VEGF蛋白的表达。结果:与模型组比较,南苜蓿总皂苷2.8 g/kg、1.4 g/kg能降低大鼠的FBG、FINS、Hb A1c水平;降低糖尿病大鼠胰腺组织MDA、IL-1β、TNF-α的水平,升高SOD、GSH-Px和肝糖原含量。2.8 g/kg组能改善糖尿病大鼠的血液流变性,减少CHOP、JNK蛋白的表达;增加VEGF蛋白的表达。结论:南苜蓿总皂苷保护T2DM大鼠胰腺的保护作用,其机制可能与抑制胰腺的氧化应激和炎症反应,减轻胰岛细胞凋亡发生,改善微循环有关。Objective： To observe the protective effects of total saponins from Medicago polymorpha（ TSMP） on pancreas of experimental T2 DM rat model. Method： Ten rats were randomly selected as the control group. The SD rats with type 2 diabetes mellitus were established,then were divided into five groups： the model group,metformin group,TSMP（ 2. 8 g/kg） and（ 1. 4 g/kg） group,which were administered for four weeks. At the end of administration,blood was collected,and fasted blood glucose（ FBG）,fasted serum insulin（ FINS）,Hb A1 c,hepatic glycogen were detected. The pancreas homogenates were used to detect the concentration of malondialdehyde（ MDA）,superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-Px）,IL-1β,TNF-α. Western blot method was adopted to determine the expressions of pancreas CHOP,JNK and VEGF protein. Results： Compared with the model group,2. 8 g/kg,1. 4 g/kg TSMP decreased levels of FBG,FINS,Hb A1c（ P〈0. 01 or P〈0. 05）. Levels of MDA,IL-1β,TNF-α were obviously decreased（ P〈0. 05）,and SOD,GSH-Px,hepatic glycogen were obviously increased compared with those in model group（ P〈0. 05）. 2. 8 g/kg TSMP reduced the whole blood viscosity,plasma viscosity,whole blood viscosity,CHOP,JNK expression levels（ P〈0. 05）. The protein expression of VEGF in pancreas tissue was significantly up-regulated（ P〈0. 05）. Conclusion： TSMP has protective effect on pancreas in T2 DM rats,the mechanism may be relate to decreasing oxidative stress、alleviating inflammatory,reducing apoptosis and improving the microcirculatory disturbance.

关 键 词：南苜蓿总皂苷 2型糖尿病 降糖 氧化应激 凋亡 

分 类 号：R285.5[医药卫生—中药学]