定清片对人白血病HL-60细胞增殖及凋亡的影响  

Impacts of Dingqing Tablets on the Proliferation and Apoptosis of HL-60 Cells in Human Leukemia

作　　者：郑丹丹[1] 孙伟玲[1] 周永明[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《世界中西医结合杂志》2017年第8期1037-1040,1100,共4页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(81373626);上海高校选拔培养优秀青年教师科研专项基金(SZY10073)

摘　　要：目的研究定清片对人白血病HL-60细胞增殖的影响及其作用机制。方法制备定清片含药血清,并以不同浓度含药血清体外干预培养HL-60细胞,MTT法检测细胞增殖抑制率,应用流式细胞术检测细胞周期及凋亡率。结果不同浓度定清片(5%、10%、20%)作用于HL-60细胞,均能抑制细胞生长,其抑制作用随药物浓度增加呈递增趋势,相同浓度组24 h、48 h、72 h抑制率递增。流式细胞术检测细胞周期和凋亡率,与正常对照组比较,定清片组G_1期细胞停滞率及凋亡率明显升高,且药物浓度越高,G_1期细胞停滞率和凋亡率越高。结论定清片能抑制人白血病HL-60细胞增殖,其作用机制可能与影响细胞周期和诱导细胞凋亡相关。Objective To explore the impacts of Chinese herbal preparation,dingqing tablets on the proliferation of HL-60 cell and its mechanism in human leukemia. Methods The drug-contained serum of dingqing tablets was prepared. With the intervention of the drug-contained serum of different concentrations,HL-60 cells were cultured in vitro. MTT assay was used to detect the inhibition rate of cell proliferation. The flow cytometry was adopted to detect the cell cycle and apoptosis rate. Results Dingqing tablets of different concentrations（ 5%,10%,20%） inhibited the growth of HL-60 cells and the inhibitory effect was getting stronger with the concentration increase,in 24 h,48 h and 72 h in the same concentration group. Compared with the blank control group,the arrest rate and apoptosis rate in G_1 phase were increased apparently in the dingqing tablets group determined in the flow cytometry. The higher the drug concentration was,the higher the arrest rate and apoptosis rate were. Conclusion Dingqing tablets inhibit HL-60 proliferation of HL-60 cell in human leukemia and its mechanism is possibly related to the impacts on the cell cycle and the induced apoptosis.

关 键 词：定清片 HL-60 细胞周期 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R733.7[医药卫生—中医学]