丹参多酚酸盐干预非ST段抬高心肌梗死患者microRNA与靶基因  

Intervention Effect of Salvianolate on microRNA and Target Genes in Patients with Non-ST-segment Elevation Myocardial Infarction

作　　者：张振鹏[1] 滕菲[1] 刘咏梅[1] 李军[1] 王阶[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中国实验方剂学杂志》2017年第19期17-22,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81473561)

摘　　要：目的:高通量检测非ST段抬高性心肌梗死(NSTEMI)患者药物干预前后的微小RNA(microRNA)表达谱,对比中西药物治疗前后microRNA表达变化,分析活血化瘀药物干预靶标与机制。方法:选取符合西医疾病诊断标准和中医证侯诊断标准的患者,分为2组,包括NSTEMI中药治疗组,NSTEMI西药组,每组纳入35例,抽取外周血,分离外周血单核细胞(PBMC),提取总RNA。根据各组差异表达的microRNA和基因的通路和网络分析结果,确定各组中起关键调控作用的microRNA及其靶基因。结果:在microRNA表达谱中,中药治疗组治疗前后、中药治疗组与西药组两组治疗后比较均存在差异;中药治疗组治疗后hsa-miR-92a,hsa-miR-363,hsa-miR-499,hsa-miR-30b,hsa-miR-454-3p,hsa-miR-933表达下调,hsa-miR-144,hsa-miR-451,hsa-miR-494,hsa-miR-320表达上调。表明这10个microRNA是丹参多酚酸盐干预NSTEMI的靶标。实时荧光定量PCR(Real-time PCR)验证,与西药组比较,中药治疗组治疗后hsa-miR-92a,hsa-miR-30b,hsa-miR-499,hsa-miR-454表达下调;中药治疗组治疗后hsa-miR-494的表达上调(P<0.05)。结论:NSTEMI患者治疗前后,中药治疗组与西药组microRNA的表达谱均存在差异,筛选的miRNA可能是活血化瘀药物干预的靶标。Objective： To conduct high throughput detection of microRNA expression in patients with non-ST segment elevation myocardial infarction （NSTEMI） before and after drug intervention, compare the expression changes of mieroRNA before and after treatment with Chinese and western medicines, and analyze the intervention targets and mechanism of Chinese medicines for promoting blood flow and eliminating blood stasis. Method： Patients who met the diagnostic criteria of Western medicine diseases and the diagnostic criteria of traditional Chinese medicine （TCM） syndromes were divided into NSTEMI Chinese medicine treatment group and NSTEMI western medicine control group, 35 cases in each group. Their peripheral blood was extracted, peripheral blood mononuclear cells （PBMC） were isolated and total RNA was extracted. The active mieroRNA and its target genes in each group were identified according to differentially expressed microRNAs, gene pathways and network analysis results. Result： In the microRNA expression profile, there were differences between Chinese medicine treatment group before and after treatment as well as between TCM treatment group and western medicine control group after treatment, hsa-miR-92a, hsa-miR-363, hsa-miR499, hsa-miR-30b, hsa-miR-454-3p, and hsa-miR- 933 expression levels were down-regulated, while hsa-miR-144, hsa-miR-451, hsa-miR-494, and hsa-miR-320 expression levels were up-regulated in TCM treatment group after treatment. These 10 microRNAs were considered as the intervention targets of salvianolate for NSTEMI. Real-time PCR, results verified that as compared with the western medicine control group, hsa-miR-92a, hsa-miR-30b, hsa-miR499 and hsa-miR454 expression levels were down-regulated while the expression of hsa-miR-494 was up-regulated after treatment in Chinese medicine treatment group （P 〈 0.05）. Conclusion： There are differences in the expression profiles of microRNAs between the Chinese medicine treatment group and the western medicine control group before and

关 键 词：非ST段抬高性心肌梗死 微小RNA 丹参多酚酸盐 治疗靶标 生物信息学 

分 类 号：R287[医药卫生—中药学] R259[医药卫生—中医学]