黄连饮片标准汤剂的制备及质量标准分析  

Preparation and Quality Standard Analysis of Coptidis Rhizoma Pieces Standard Decoction

作　　者：崔文金 焦梦姣 邓哲[1,2] 章军[2] 冯伟红[2] 刘安[2] 

机构地区：[1]江西中医药大学,南昌330004 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2017年第19期40-45,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院"十三五"重点领域研究专项(zz10-007)

摘　　要：目的:制备黄连饮片标准汤剂并建立其质量标准,为黄连配方颗粒的制备及其质量标准的建立提供参考。方法:根据中药饮片标准汤剂制备原则制备黄连饮片标准汤剂,计算表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的转移率和饮片出膏率,建立黄连饮片标准汤剂的质量标准。流动相乙腈-0.05 mol·L^(-1)磷酸二氢钾溶液(50∶50)(每100 m L中加十二烷基硫酸钠0.4 g,以磷酸调节pH 3.0),柱温30℃,流速1 mL·min^(-1),检测波长225 nm。结果:黄连饮片中表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的转移率范围分别为79.3%~111.9%,54.6%~76.2%,45.7%~70.7%和43.5%~64.4%,出膏率范围17.1%~22.3%,14批黄连饮片标准汤剂特征图谱中有7个共有峰,其相似度均>0.999。结论:该研究建立的质量评价方法精密度和重复性良好,指纹图谱相似度高,适用于黄连饮片标准汤剂的质量评价。Objective： To prepare Coptidis standard. Method： According to the standard of Coptidis Rhizoma pieces standard decoction was Rhizoma pieces standard decoction and establish its quality Chinese herbal medicine decoction preparation principle, prepared, the transfer rates of epiberberine, coptisine hydrochloride, palmatine hydrochloride and berberine hydrochloride and the extract rate were calculated. The quality standard of this standard decoction was also established. Result： The transfer rates of epiberberine, coptisine hydrochloride, palmatine hydrochloride and berberine hydrochloride were 79.3%-111.9% , 54.6%- 76.2% , 45.7% -70.7% and 43.5% -64.4% , respectively; and the extract rate was 17.1% -22.3%. Moreover, 7 common peaks were determined in 14 batches of Coptidis Rhizoma pieces standard decoction. The similarities of samples were all higher than 0. 999. Conclusion： The method displays good precision, stability and repeatability in fingerprint analysis, which is suitable for the quality evaluation of Coptidis Rhizoma pieces standard decoction.

关 键 词：黄连 标准汤剂 饮片 指纹图谱 表小檗碱 黄连碱 巴马汀 小檗碱 

分 类 号：R283.6[医药卫生—中药学] R284[医药卫生—中医学]