小檗碱对PCOS模型大鼠子宫内膜的影响  

Effect of Berberine on the Endometria of the PCOS Rats

作　　者：刘逸超[1,2] 付杨 郭滢[1] 郭琦[1] 韩凤娟[3] LIU Yi-chao FU Yang GUO Ying GUO Qi HAN Feng-juan(Heilongfiang University of Chinese Medicine, Harbin 150040, China The Second Hospital of Harbin, Harbin 150056 China The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]哈尔滨市第二医院,黑龙江哈尔滨150056 [3]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中医药信息》2017年第5期16-19,共4页Information on Traditional Chinese Medicine

基　　金：国家中医药管理局国家中医临床研究基地业务建设科研专项课题(No.JDZX2012038)

摘　　要：目的:探讨小檗碱对PCOS模型大鼠血清睾酮、子宫内膜相关基因及蛋白信号的影响。方法:90日龄清洁级SD雌性大鼠根据Poretsky造模方法皮下注射胰岛素及绒毛膜促性腺激素,连续22日构建PCOS模型。小檗碱灌胃治疗28日,采用ELISA法检测睾酮激素水平,采用免疫组化法检测子宫内膜组织ERα蛋白表达情况,qRT-PCR技术检测子宫内膜Kras、PTEN mRNA基因表达水平。结果:与模型组相比,小檗碱组睾酮水平显著降低(P<0.05);ERα阳性表达率下降(P<0.05);Kras、PTEN mRNA表达水平下降(P<0.05)。结论:小檗碱对PCOS模型大鼠子宫内膜有显著影响,对激素合成、细胞癌变等信号分子具有调控作用。Objective：To explore the influence of Berberine on testosterone level and the expressions of endometrial gene and its protein in rats with polycystic ovarian syndrome （PCOS）.Methods：120 female Sprague-Dawley rats of 90 days were made into PCOS models with 22-day subcutaneous injection of insulin and human chorionic gonadotropin（HCG）.After modeling,Berberine was given to rats in the next 28 days.The testosterone levels was tested by ELISA method;the protein expression of endometrial estrogen receptor α（Era） was detected with immunohistochemical method;the expression levels of endometrial Kras and PTEN mRNA gene were detected with qRT-PCR technique.Results：Compared to the model group,the testosterone level in Berberine group was significantly decreased（P〈0.05）;the positive protein expression of Era,and the gene expressions of Kras and PTEN mRNA were significantly reduced in endometrial tissue（P〈0.05）.Conclusion：Berberine has significant effects on the endometria of PCOS rats,which regulates the hormone synthesis and cell carcinoma.

关 键 词：多囊卵巢综合征 子宫内膜癌 小檗碱 

分 类 号：R285.5[医药卫生—中药学]