嗜线虫致病杆菌属Pir毒素基因克隆和生物信息学分析  

Cloning and bioinformatics analysis of Pir toxins genes from Xenorhabdus nematophila

作　　者：杨晴[1] 张杰 李天慧[1] 南宫自艳[1] 王勤英[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071000 [2]河北省承德市滦平国有林场管理处,河北滦平068250

出　　处：《中国植保导刊》2017年第9期5-11,共7页China Plant Protection

基　　金：河北省自然科学基金(C2016204107);河北省现代农业产业技术体系棉花创新团队(HBCT2013030201)

摘　　要：采用基因组DNA提取法和目的基因PCR扩增对嗜线虫致病杆菌(Xenorhabdus nematophila)HB310(Xn HB310)菌株中的pirA和pirB基因进行克隆及生物信息学分析。结果表明,pirA全长408 bp,pirB全长1 290 bp;其蛋白PirA分子量为14.9 kDa,含135个氨基酸,PirB分子量为48.2 kDa,含429个氨基酸;二级结构分析表明PirA无α-螺旋,含12个β折叠,PirB含8个α螺旋和12个β折叠;亚细胞定位表明PirA和PirB主要分布在细胞核;PirA不含跨膜结构域和信号肽,PirB含有跨膜结构域和凝集素结构域,不含信号肽。In this paper,pirA and pirB genes of Xenorhabdus nematophila HB310 strains （Xn HB310） were cloned. The results showed that full length of pirA and pirB was 408bp and 1290bp respectively. PirA was a protein of 14.9 kDa, which was composed of 135 amino acids. PirB was about 48.2 kDa, which was composed of 429 amino acids. PirA had 12 β-pleated sheets but no α-helix. PirB contained 8 α-helix and 12 β-pleated sheets. PirA and PirB were mainly distributed in nucleus. Neither of PirA and PirB contained signal peptide, while PirB contained transmembrane domain and a Jacalin- like super family domain. These results provided a theoretical basis for revealing pir gene function and genetic character- istics of Xenorhabdus.

关 键 词：嗜线虫致病杆菌 Pir毒素 基因克隆 序列分析 生物信息学分析 

分 类 号：S432.45[农业科学—植物病理学] Q785[农业科学—农业昆虫与害虫防治]