深圳市H5N6、H7N9流感病毒HA-UTR基因分子特征分析  

Molecular characteristics of the untranslated segment-specific region of the hemagglutinin segments in H5N6 and H7N9 influenza viruses in Shenzhen

作　　者：陈燕慈[1] 王昕[2] 彭博[2] 武伟华[2] 刘慧[2] 耿艺介[2] 郑青[1] 房师松[2] Chen Yanci Wang Xin Peng Bo Wu Weihua Liu Hui Geng Yijie Zheng Qing Fang Shisong(College of Pharmacy, Jinan University, Guangzhou 510632, China（ Chen YC, Zheng Q Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China（ Wang X, Peng B, Wu WH, Liu H, Geng YJ, Fang SS)

机构地区：[1]暨南大学药学院,广州510632 [2]深圳市疾病预防控制中心,518055

出　　处：《中华实验和临床病毒学杂志》2017年第4期291-294,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：深圳市卫生计生系统科研项目（201602002）

摘　　要：目的 了解深圳市H5N6、H7 N9流感病毒HA基因非翻译区（Untranslated region,UTR）的分子特征.方法 使用MEGA7.0、DNAStar7.1.0等生物信息学分析软件对全球及深圳H5N6、H7N9流感病毒HA基因的UTR进行核苷酸序列同源性分析及基因多态性分析.结果 3株2014-2015年深圳市H5N6病毒与其他H5NX病毒株比较,HA-3＇UTR核苷酸同源性为77.4％-100％,H5N6-HA-3＇UTR未见明显突变位点;HA-5＇UTR核苷酸同源性为91.7％ - 100％,H5N6-HA-5＇UTR第24、31位发生突变.11株2013-2014年深圳市H7N9病毒与其他H7NX病毒株比较,HA-5＇UTR核苷酸同源性为81.2％ -100％,H7N9-HA-5＇UTR在第2-6、9、10、12及15 - 17位发生多位点突变.结论 深圳市人感染H5N6及H7N9病毒HA的UTR保守区高度同源性及其可变区具有基因多态性.Objective Exploring the molecular characteristic of global and Shenzhen district H5N6 and H7N9 influenza viruses HA untranslated regions （UTRs）.Methods Mega7.0 and DNAStar 7.1.0 were used to construct phylogenetic tree and nucleotide analysis.Results From 2014 to 2015,3 strains of H5N6 influenza virus from Shenzhen were compared with the other H5NX influenza viruses,the nucleotide homology of HA-3＇UTR was 77.4%-100%,which did not have obvious mutated sites.The nucleotide homology of H5N6-HA-5＇UTR was 91.7%-100%,and the sites of 24 and 31 sites were mutated.From 2013 to 2014,11 strains of H7N9 influenza virus from Shenzhen were compared with the other H7NX influenza viruses,the nucleotide homology of H7N9-HA-5＇UTR was 76.8%-100%,which had multimutated sites on 2-6,9,10,12 and 15-17 positions.Conclusions HA-UTR from human-infected H5N6 and H7N9 influenza viruses isolated in Shenzhen district has unique molecular characteristics,its conserved region has relatively high homology and the segment-specific region has genetic polymorphism.

关 键 词：H5N6流感病毒 H7N9流感病毒 血凝素 非翻译区 同源性 多态性 

分 类 号：R373[医药卫生—病原生物学]