2015年甘肃省外环境禽流感病毒监测预警分析  

Early warning analysis of avian influenza virus in environmental samples in Gansu province,2015

作　　者：李保娣[1] 李红育[1] 张慧[1] 刘丽琦[2] 李梓[2] 于德山[1] 何健[1] Li Baodi Li Hongyu Zhang Hui Liu Liqi Li Zi Yu Deshan He Jian(Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730000, China（ Li BD, Li HY, Zhang H, Yu DS, He J Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China（ Liu LQ, Li Z)

机构地区：[1]甘肃省疾病预防控制中心,兰州730000 [2]中国疾病预防控制中心病毒病预防控制所国家流感中心,北京102206

出　　处：《中华实验和临床病毒学杂志》2017年第4期307-310,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家“十二五”艾滋病和病毒性肝炎等重大传染病防治科技重大专项（2012ZX10004-208）;甘肃省卫生行业科研计划项目（GSWSKY2017-28）

摘　　要：目的 了解甘肃省外环境中禽流感病毒H5、H7、H9亚型的分布状况,科学指导全省禽流感防控工作.方法 采用实时荧光定量聚合酶链反应法（Real-time fluorescence quantitativePolymerase Chain Reaction,real-time PCR）对2015年全省14个市州采集的标本2 015份进行禽流感病毒核酸检测.结果 FluA、H5、H7、H9的检出率依次为：19.90％、0.05％、0和19.65％.FluA检出率最高的环境标本为宰杀或摆放禽肉案板表面的擦拭标本（31.09％）,最低的为粪便标本（13.85％）.FluA检出率在12月份最高（28.18％）,6、7月份最低（8.59％和8.66％）.定西市和陇南市禽流感病毒核酸FluA和H9的检出率显著高于其他市州（χ^2=4.523 - 88.059,P=0.000-0.043〈0.05）.结论 2015年甘肃省涉禽外环境中存在的禽流感病毒污染主要为H9亚型,未检出H7N9,应加强监测,做好禽流感预警.Objective To understand the contamination status of avian influenza virus H5,H7,and H9 in environmental samples in Gansu province during 2015,and provide scientific guidance for the prevention and control of human infection with AIV in the province.Methods We detected the AIV nucleic acid of 2015 environmental samples with real-time PCR;the samples were collected from all of the monitoring points in the whole province,then we analyzed the result of environmental monitoring of pathogens in the province.Results The detection rates of FluA,H5,H7 and H9 were 19.90%,0.05%,0 and 19.65% respectively.The sample specific detection rate of FluA was the highest in wipe specimen of the board surface of slaughtering or placing poultry （31.09%） and the lowest in stool specimens （13.85%）.FluA detection rate was the highest in December and the lowest in June and July （8.59% and 8.66%）.The detection rate of Dingxi city and Longnan city of nucleic acid of avian 2influenza virus FluA and H9 were significantly higher than those in other cities in the province（χ^2=4.523- 88.059,P 〈 0.05）.Conclusions The main AIV pollution of the external environment in Gansu province in 2015 were the H9 subtype,and there was no H7N9 positive,we should strengthen the monitoring and early warning for avian influenza.

关 键 词：禽流感病毒 聚合酶链反应 监测 

分 类 号：R181.8[医药卫生—流行病学] R511.7[医药卫生—公共卫生与预防医学]