2012年云南省边境地区蚊虫及蚊传虫媒病毒调查  

Investigation of mosquitoes and arboviruses in the border areas of Yunnan province, 2012

作　　者：雷雯雯[1] 郭晓芳[2] 付士红[1] 冯云[3] 杨中华[2] 王环宇[1] 何英[1] 高晓艳[1] 吕志[1] 周红宁[2] 梁国栋[1] Lei Wenwen Guo Xiaofang Fu Shihong Feng Yun Yang Zhonghua Wang Huanyu He Ying Gao Xiaoyan Lyu Zhi Zhou Hongning Liang Guodong(State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China （ Lei WW, Fu SH, Wang HY, He Y, Gao XY, Lyu Z, Liang GD Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Institute of Parasitic Diseases, Pu＇ er 665000, China （ Guo XF, Yang ZH, Zhou HN Yunnan Institute of Endemic Disease Control and Prevention, Yunnan Provincial Center of Virus and Rickettsia Research, Dali 671000, China （ Feng Y)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京102206 [2]云南省寄生虫病防治所云南省虫媒传染病防控研究重点实验室,普洱665000 [3]云南省地方病防治所云南省病毒立克次体研究中心,大理671000

出　　处：《中华实验和临床病毒学杂志》2017年第4期311-314,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金（81290342）;传染病预防控制国家重点实验室项目（2014SKLID103）

摘　　要：目的 了解云南省南部边境地区蚊虫中虫媒病毒的分布特点,为虫媒病毒病防治提供科学依据.方法 2012年7-8月在云南省西双版纳傣族自治州勐海县打洛镇和普洱市江城县整董镇采集蚊虫标本,用组织培养法分离病毒,使用蚊媒病毒种属特异性引物对阳性病毒分离物进行鉴定.结果 共采集蚊虫标本6属17种18714只.三带喙库蚊为当地优势蚊种,占蚊虫采集总数的59.74％（11 179/18 714只）.从采集的蚊虫标本中分离到23株病毒分离物,其中2株坦布苏病毒（Tembusu virus,TMUV）、3株乙脑病毒（Japanese encephalitis virus,JEV）、2株盖塔病毒（Getah virus,GETV）、4株版纳病毒（Banna virus,BAV）、9株浓核病毒（Densovirus,DNV）和3株套式病毒（Nam Dinh virus,NDiV）.结论 云南省边境地区不仅蚊虫种类丰富,而且蚊虫携带多种虫媒病毒.Objective To investigate the distribution patterns of arboviruses in Yunnan province near the China-Laos-Myanmar border,China,and to provide evidence for prevention and control of arboviruses diseases.Methods Mosquito samples were collected in Daluo county of Xishuangbanna Dai Autonomous Prefecture and Zhengdong county of Pu＇er city in Yunnan province,2012.Viruses were isolated from the samples by tissue culture,positive isolates were identified by RT-PCR with arbovirus species-specific primers,for further sequencing and phylogenetic analysis.Results A total of 17 species of mosquitoes from 6 genera were collected.A total of 24 strains of viruses were isolated frown the mosquito pools and identified as Tembusu virus （TMUV） （2 strains）,Japanese encephalitis virus （JEV） （3 strains）,Getahvirus （GETV） （2 strains）,Banna virus （BAV） （4 strains）,Densovirus （DNV） （9 strains） and Nam Dinh virus （NDiV） （3 strains）.Conclusions The China-Laos-Myanmar border of Yunnan province is rich in species of mosquitoes and arboviruses.

关 键 词：虫媒病毒 云南省 调查 

分 类 号：R181.3[医药卫生—流行病学]