水蛭提取物结肠迟释微丸与普通微丸的抗血栓作用比较  

Comparison of Antithrombotic Effect between Colon Delayed-release Pellets and Regular Pellets of Whitmania pigra Whitman Extract

作　　者：周成浩 陈文培[1] 张子扬[1] 姚小兰 林俊粒 关世侠[1] 林宝琴[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广东莱恩医药研究院有限公司,广东广州510990

出　　处：《中药新药与临床药理》2017年第5期568-572,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：基金项目:广东省科技厅公益研究与能力建设(2016A020217016)

摘　　要：目的比较水蛭提取物结肠迟释微丸(colon delayed-release pellets,CDP)和水蛭提取物普通微丸(regular pellets,RP)的抗凝血和抗血栓形成作用。方法采用毛细管法检测新西兰兔凝血时间,考察CDP和RP对大鼠动-静脉旁路血栓、狭窄法诱导的下腔静脉血栓和三氯化铁(FeCl_3)诱导颈动脉血栓形成的作用。凝血时间测定实验为单次给药,设3个组:正常组,CDP组(64 mg·kg^(-1)·d^(-1))和RP组(43.7 mg·kg^(-1)·d^(-1));动-静脉旁路血栓实验为单次给药,设6个组:模型组,CDP低、高剂量组(87,174 mg·kg^(-1)·d^(-1)),RP低、高剂量组(59.4,118.8mg·kg^(-1)·d^(-1)),阿司匹林组(7.8 mg·kg^(-1)·d^(-1));狭窄法诱导大鼠下腔静脉血栓实验设8组:假手术组,模型组,CDP低、高剂量组,RP低、高剂量组,阳性药华法林组(2.0 mg·kg^(-1)·d^(-1))和活血通脉胶囊组(312.5 mg·kg^(-1)·d^(-1)),每天给药1次,连续给药7 d;FeCl_3诱导颈动脉血栓实验为单次给药,设8组:假手术组,模型组,CDP低、高剂量组,RP低、高剂量组,阳性药氯吡格雷组(30 mg·kg^(-1)·d^(-1))和活血通脉胶囊组。称量动-静脉旁路血栓湿重和干重以及静脉血栓质量和长度,计算血栓形成抑制率;对动脉血栓进行半定量病理学分析,计算血栓形成百分比和血栓形成抑制率。结果与正常组比较,CDP组和RP组分别在给药后4 h和0.5 h显著延长新西兰兔凝血时间(P<0.05),抗凝作用分别维持4 h和1 h。CDP组和RP组能剂量依赖性抑制大鼠动-静脉旁路血栓和下腔静脉血栓形成,抑制动脉血栓形成,与模型组比较,差异有统计学意义(P<0.01)。此外,在抑制大鼠动-静脉旁路血栓和下腔静脉血栓作用上,CDP强于RP。结论 CDP抗血栓形成作用显著优于RP,且CDP抗静脉血栓的作用比抗动脉血栓明显。Objective The aim of the study was to compare anticoagulability and antithrombotic effects of colon delayed-release pellets （CDP） of Whitmania pigra Whitman extract with corresponding regular pellets （RP）. Methods Capillary tube method was applied to detect coagulation time in rabbits. Antithrombotic effects were evaluated by arteriovenous（AV） shunt-induced thrombosis, inferior vena eava（IVC） ligation-induced thrombosis and ferric chloride （FeCls）-induced arterial thrombosis in rats. In the study of coagulation time, rabbits were randomly divided into three groups： normal group, RP group （43.7 mg·kg^-1·d^-1） and CDP group （64 mg·kg^-1·d^-1）. In the experiment of AV shunt-induced thrombosis, rats were randomly divided into six groups： model group, two dosages of RP group （59.4, 118.8 mg·kg-l·d-I） , two dosages of CDP group（87, 174 mg·kg^-1·d^-1） and aspirin group（7.8 mg·kg^-1·d^-1）. In experiment of IVC ligation-induced thrombosis, rats were randomly divided into eight groups： Sham group, model group, two dosages of RP group, two dosages of CDP group, warfarin group（20 mg·kg^-1 ·d^-1） and Huoxue Tongmai Jiaonang group （312.5 mg·kg^-1· d^-1）. In experiment of FeC13-induced thrombosis, rats were randomly divided into eight groups： Sham group, model group, two dosages of RP group, two dosages of CDP group, clopidogrel group （30 mg·kg^-1· d^-1） and Huoxue Tongmai Jiaonang group. Wet weight and dry weight of thrombus were measured and the rate of thrombosis inhibition percentage were calculated, arterial thrombosis were evaluated by histological analysis. Results CDP and RP significantly prolonged coagulation time at 4 h and 0.5 h after administration（P 〈 0.05 vs. normal group）, and the effect maintained for 4 h and 1 h, respectively. CDP and RP dose-dependently inhibited AV shunt- and IVC ligation-induced thrombosis, and reduced FeC13-induced thrombosis formation. The antithrombotic effect of CDP was better than RP. Conclusion CDP of Whi

关 键 词：宽体金线蛭 结肠迟释微丸 凝血时间 动-静脉旁路血栓 下腔静脉血栓 动脉血栓 

分 类 号：R285.5[医药卫生—中药学]