鸡骨草叶黄酮类成分HPLC指纹图谱研究  

Study on HPLC Fingerprints of Flavonoids from Abri Herba Leaves

作　　者：袁旭江 霍务贞 鲁湘鄂 吴燕红 

机构地区：[1]广东药科大学中药开发研究所/国家中医药管理局三级实验室,广东广州510006

出　　处：《中药新药与临床药理》2017年第5期673-677,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省自然科学基金博士启动项目(2016A030310302);广东省科学技术厅-广东中医药科学院科研联合项目(2014A020221105)

摘　　要：目的建立鸡骨草叶黄酮类HPLC指纹图谱,分析鸡骨草叶及饮片品质,为鸡骨草质控提供依据。方法采用RP-HPLC法,色谱柱为Agilent ZORBAX SB-C_(18)(250 mm×4.6 mm,5μm);流动相为甲醇-0.2%乙酸,梯度洗脱;检测波长:330 nm;流速:1.0 mL·min^(-1)。建立鸡骨草叶黄酮类HPLC指纹图谱,进行相似度和聚类分析,并对鸡骨草饮片品质进行评价。结果建立了15批鸡骨草叶黄酮类HPLC指纹图谱,标记出10个特征共有峰,峰面积总和>95%。不同样品相似度存在一定差异,第Ⅰ类鸡骨草叶,产地较近,相似度>96;第Ⅱ类鸡骨草叶,产地较远,相似度0.32~0.84;第Ⅲ类为毛鸡骨草叶,产地接近第Ⅰ类,相似度0.85~1.00;20批饮片样品HPLC指纹图谱相似度在0.58~0.96之间。结论产地、基源和部位均影响着鸡骨草品质;所建黄酮类HPLC指纹图谱法符合指纹图谱的要求,可用于鸡骨草叶、鸡骨草饮片的质量评价。Objective To establish the HPLC fingerprints of the flavonoids from Abri herba leaves, and carry out the quality evaluation of Abri herba leaves and prepared slices to lay the foundation for quality control of Abri herba. Methods An RP-HPLC method was used with the chromatographic column of Agilent ZORBAX SB-CIs（250 mm × 4.6 mm, 5 μm）, the mobile phase of methanol-0.2 % acetic acid by gradient elution, the detection wavelength at 330 nm, and the flow rate of 1 mL· min^-1. The HPLC fingerprints for flavonoids from leaves of Abri herba were determined, similarity comparison and cluster analysis were also conducted. The prepared slices of Abri herba were determined for flavonoids HPLC fingerprints and quality evaluation. Results The flavonoids HPLC fingerprints of 15 bathes of Abri herba leaves were established. Ten stable characteristic common peaks were assigned with the total peak area larger than 95 %. According to the similarity evaluation and cluster analysis, differences between different samples were shown： the first class （ Ⅰ） of A brus cantoniensis leaves, whose places of origin were close to each other, with a similarity higher than 0.96; the second class （Ⅱ） of Abrus cantoniensis leaves, whose places of origin were far from each other with a similarity between 0.32 - 0.84; the third class（Ⅲ） was A brus mollis leaves, whose places of origin were close to class I with the similarity between 0.86-1.00. Furthermore, 20 batches of Abri herba prepared slices showed the similarities between 0.58-0.96. Conclusion The quality of Abri herba is affected by the place of origin, source and medicinal part. The method of HPLC fingerprint analysis of flavonoids conforms to the requirements of fingerprint and can be used to evaluate the quality of Abri herba leaves and prepared slices.

关 键 词：鸡骨草 毛鸡骨草 黄酮类 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]