Diversity and bioactivity of cultured aquatic fungi from the High Arctic region  

作　　者：ZHANG Tao ZHAO Lili YU Caiyun WEI Tao YU Liyan 

机构地区：[1]China Pharmaceutical Culture Collection,Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences& Peking Union Medical College [2]Beijing Key Laboratory of Bioactive Substances and Function Food,Beijing Union University

出　　处：《Advances in Polar Science》2017年第1期29-42,共14页极地科学进展（英文版）

基　　金：supported by the National Natural Science Foundation of China (NSFC) (Grant nos.31670025 and 31300115);Projects of the Chinese Arctic and Antarctic Administration,State Oceanic Administration (Grant no.2013YR06006);the National Infrastructure of Microbial Resources (Grant no.NIMR-2017-3);the CAMS Innovation Fund for Medical Sciences (Grant no.2016-I2M-2-002)

摘　　要：This study assessed the diversity and α-glycosidase inhibitory activity of cultured fungi isolated from four aquatic environments （stream, pond, glacial ice, and estuary） in the Ny-A.lesund region （Svalbard, Norway, High Arctic）. A total of 134 fungal isolates were obtained from 13 water samples. Based on morphological characteristics and sequence analyses of the nuclear ribosomal DNA internal transcribed spacer region, these fungal isolates were identified as belonging to 47 species, with 26 belonging to the Ascomycota, 20 to the Basidiomycota, and one to the Zygomycota. The most frequently detected fungal species were Vishniacozyma sp. 2, Cadophora sp. 2, Phenoliferia sp. 1, Dioszegia sp. 2, and Mortierella sp.; these species occurred in 10, eight, seven, six, and five of the samples, respectively. Among the 134 fungal isolates, 17 isolates of 15 species displayed high α-glycosidase inhibitory activity in culture. The results suggest that diverse and distinct populations of cultured fungi are present in Arctic aquatic environments, and they include taxa that are potential sources of bioactive molecules that may be used as prototype drugs for medicinal proposals.This study assessed the diversity and α-glycosidase inhibitory activity of cultured fungi isolated from four aquatic environments （stream, pond, glacial ice, and estuary） in the Ny-A.lesund region （Svalbard, Norway, High Arctic）. A total of 134 fungal isolates were obtained from 13 water samples. Based on morphological characteristics and sequence analyses of the nuclear ribosomal DNA internal transcribed spacer region, these fungal isolates were identified as belonging to 47 species, with 26 belonging to the Ascomycota, 20 to the Basidiomycota, and one to the Zygomycota. The most frequently detected fungal species were Vishniacozyma sp. 2, Cadophora sp. 2, Phenoliferia sp. 1, Dioszegia sp. 2, and Mortierella sp.; these species occurred in 10, eight, seven, six, and five of the samples, respectively. Among the 134 fungal isolates, 17 isolates of 15 species displayed high α-glycosidase inhibitory activity in culture. The results suggest that diverse and distinct populations of cultured fungi are present in Arctic aquatic environments, and they include taxa that are potential sources of bioactive molecules that may be used as prototype drugs for medicinal proposals.

关 键 词：aquatic habitats bioactivity DIVERSITY environmental mycology High Arctic 

分 类 号：Q93[生物学—微生物学]