miR-34a在当归补血汤抗小鼠心肌梗死后心室重塑中的表达  

Expression of miR-34a in the myocardium of mice received Danggui Buxue Decoction therapy following myocardial infarction

作　　者：范智文[1] 赵一俏[2] 凌龙[1] 席玉胜[1] 靳文[2] 贺不凡 曾伟杰[1] 

机构地区：[1]中国人民解放军第421医院心内科,广州市510318 [2]广东省第二人民医院心内科,广州市510318

出　　处：《实用医学杂志》2017年第18期3009-3012,共4页The Journal of Practical Medicine

基　　金：广东省中医药局科研课题(编号:20151041);广东省自然科学基金(编号:2016A030313853);解放军第421医院医学创新项目(编号:201604)

摘　　要：目的探讨当归补血汤抗小鼠心肌梗死后心室重塑以及对miR-34a表达的影响。方法采用左冠状动脉前降支近端结扎法构建小鼠心肌梗死模型后分为当归补血汤组和模型组。当归补血汤组予以当归补血汤灌胃,模型组予以等容量生理盐水灌胃,连续给药8周。后心脏超声检查小鼠左室射血分数,TUNEL染色法检测心肌细胞凋亡,Masson染色检测心肌纤维化,定量PCR检测心肌miR-34a表达,蛋白印迹检测Sirt1表达。结果与模型组相比,当归补血汤组小鼠EF值获得明显改善(P<0.05)、心肌细胞指数减少(P<0.05)、胶原纤维胶原含量降低(P<0.05)、心肌组织miR-34a表达降低以及Sirt1表达升高(P<0.05)。结论当归补血汤能够有效抑制小鼠心肌梗死后心室重塑,与抑制miR-34a、促进Sirt1表达有关。Objective To investigate the effect of Danggui buxue decoction on ventricular remodeling and miR-34a expression after myocardial infarction in mice. Methods Mice myocardial infarction model were estab- lished by the left anterior descending coronary artery embolization. Then the mice were divided into Danggui buxue Decoction group and model group. Mice in Danggui buxue decoction group were given Danggui buxue decoction, and mice in the model group were given the equal volume of saline for 8 weeks. Cardiomyocyte apoptosis was detect- ed by TUNEL staining. Myocardial fibrosis was detected by Masson staining. The expression of miR-34a was detect- ed by quantitative PCR and the expression of Sirtl was detected by Western blot assay. Results Compared with the model group, the EF value of the mice was significantly improved （P 〈 0.05 ） , the myocardial cell index decreased （P 〈 0.05）, the content of collagen fibers decreased （P 〈 0.05）, the expression of miR-34a decreased, but the expression of Sirtl increased （P 〈 0.05） in Danggui buxue Decoction group. Conclusion Danggui buxue decoction can effectively inhibit ventricular remodeling of mice following myocardial infarction, which is associated with the suppression of miR-34a and the increase of Sirt 1.

关 键 词：心室重塑 当归补血汤 MIR-34A SIRT1 

分 类 号：R285.5[医药卫生—中药学]