盐胁迫对盐芥硝酸盐的吸收及根系生长发育的影响  

Effects of Salt Stress on Absorption of Nitrate and Root Growth and Development in Eutrema salsuginea

作　　者：姜娜娜[1,2] 崔凤 刘译阳[1] 韩燕[1] 张荃[3] 李军[1] 李国卫[1] 

机构地区：[1]山东省农业科学院生物技术研究中心/山东省作物遗传改良与生态生理重点实验室,山东济南250100 [2]山东种业集团股份有限公司,山东济南250100 [3]山东师范大学生命科学学院,山东济南250014

出　　处：《山东农业科学》2017年第9期37-41,共5页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2014YL043;ZR2014CQ046);山东省农业科学院科技创新工程项目(CXGC2016C03);山东省农业良种工程项目;山东省泰山学者人才工程项目

摘　　要：氮素是植物生长发育所必需的主要营养元素,硝酸盐是植物吸收氮的主要形式。盐胁迫是一类非生物胁迫,抑制植物的生长发育。为了探讨盐胁迫影响硝酸盐吸收与植物生长发育的关系,本研究以耐盐模式植物盐芥为试验材料,测定了盐胁迫下盐芥体内硝酸盐含量的变化,分析其与根系发育的关系,并利用RNA-seq技术分析了盐胁迫条件下盐芥中硝酸根转运蛋白(nitrate transporter,NRT)基因的表达调控。结果显示,盐胁迫后盐芥叶片和根中的硝酸盐含量均显著下降,且根系干重与硝酸盐含量呈正相关关系,表明盐胁迫抑制了盐芥对硝酸盐的吸收,并且影响根系的发育。RNA-seq共检测到10个盐芥硝酸根转运蛋白基因的表达,大部分基因的表达受盐胁迫的调控,可能参与了盐胁迫条件下盐芥体内硝酸盐的吸收和转运。综合上述结果,推测盐胁迫抑制盐芥对硝酸盐的吸收,可能是导致盐胁迫下植物生长发育受抑制的因素之一。Nitrogen is a major nutrient element necessary to plant growth and development,and nitrate is the main form of nitrogen absorbed by plants. Salt stress is one type of abiotic stress,which defects plant growth and development. In order to study the effects of salt stress on nitrate absorption and plant growth and development,we employed salt-tolerant model plant Eutrema salsuginea as materials. The changes of nitrate concentrations in Eutrema salsuginea under salt stress were measured,and its relationship with root development was analyzed. In addition,the expression levels of nitrate transporter（ NRT） genes were analyzed using RNA-seq technology under salt stress conditions. The results showed that the nitrate contents in leaves and roots were both significantly decreased after salt stress treatment,and the root dry weight had a positive correlation with nitrate content. It implied that salt stress inhibited plant nitrate absorption,and further affected the growth of root system. Totally,ten NRTs were detected by RNA-seq analysis,and the expression of most of NRTs was regulated by salt stress. It suggested that these genes might be related with nitrate absorption andtransport in Eutrema salsuginea under high salt environment. In conclusion,we presume that salt stress inhibits the nitrate absorption in Eutrema salsuginea,which might be one of the factors limiting the growth and development of plants under salt stress.

关 键 词：盐芥 盐胁迫 硝酸盐吸收 硝酸根转运蛋白 

分 类 号：S580.1[农业科学—作物学]