宝鸡市不同小麦品种水培叶片抗性指标差异分析  

The difference analysis of the leaf resistance index of different wheat varieties in Baoji City

作　　者：张辉[1] 张蓓蓓[1] 

机构地区：[1]宝鸡文理学院陕西省灾害监测与机理模拟重点实验室,陕西宝鸡721013

出　　处：《宝鸡文理学院学报（自然科学版）》2017年第3期57-60,71,共5页Journal of Baoji University of Arts and Sciences(Natural Science Edition)

基　　金：国家自然科学基金项目(41601016);陕西省科技厅自然基金(2017JQ4023);宝鸡市科技计划项目(16RKX1-14);宝鸡文理学院硕士科研启动项目(ZK2017070)

摘　　要：目的了解宝鸡市不同小麦品种的抗逆性,筛选该区适合种植的小麦品种。方法选取11个冬小麦品种的种子为研究对象,以丙二醛(MDA)含量、过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性为抗逆性指标进行分析,最后对这几个指标进行相关分析。结果 (1)不同品种小麦叶片MDA含量差异显著(F=32.29,P<0.01),以宝研85叶片的MDA含量最高,为22.95 mmol·g^(-1)FW,以周麦27和荔高2号MDA含量较低,分别为4.91和4.86mmol·g^(-1) FW;(2)不同品种小麦叶片过氧化物酶(POD)活性之间达到极显著差异(F=77.78,P<0.01),其中,周麦27叶片的POD活性最强,为32.67U/min·g FW,宝研85叶片的POD活性最弱,为11.53U/min·g FW;(3)不同品种小麦叶片超氧化物歧化酶(SOD)活性也达到极显著差异(F=15.83,P<0.01),以周麦27叶片的超氧化物歧化酶活性最强,其值为43.76U/g FW,活性值最低的品种是宝研85,其值为39.21U/g FW;(4)不同品种间MDA含量与POD及SOD活性均呈现显著的负相关关系,相关系数分别为-0.75和-0.83,POD与SOD呈现显著的正相关关系,相关系数为0.68。结论宝鸡市主栽的11个小麦品种以周麦27的抗性最强,宝研85抗性较弱。Purposes--To understand the stress resistance of different wheat varieties in Baoji Cit- y, Shaanxi, China and to screen the wheat varieties suitable for being grown in the area. Methods-- The seeds of 11 winter wheat varieties in Baoji City were selected as the research objects and were ana- lyzed with the malondialdehyde （MDA） content as well as peroxidase （POD） and superoxide dis- mutase （SOD） as the indices of resistance, then the relationships among these three indices were also investigated. Results--（1） There was more significant difference in the leaf MDA content among dif- ferent wheat varieties （F=32.99, P〈0.01）, the highest MDA content, 22.95 mmol· g^1 FW, was in Baoyan 85% the lowest MDA content was in Zhoumai 27 and Ligao 2, their value was 4.91 and 4.86 mmol·g^-1 FW, respectively; （2） There was more significant difference in leaf POD activities among different wheat varieties （F=77.78, P《0.01）. The strongest POD activity, 32.67 U/min ·g FW,was in Zhoumai 27% the lowest value of activity, 11.53 U/min · g FW, was in Baoyan 85%（3） There was also more significant differences in the SOD activity （F= 15.83, P〈0.01） among 11 wheat varie- ties, and the Zhoumai 27 had the highest superoxide dismutase activity, 43.76 U/g FW, while the lowest activity, 39.21 U/g FW, was in Baoyan 85% （4） There were significant negative correlations between MDA content and POD, SOD activities in different variety seeds, and the correlation coeffi- cient was -0.75 and -0.83, separately, while the relationship between POD and SOD was positive significant and the correlation coefficient was 0.68. Conclusio^Among the 11 main wheat varieties in Baoji City, Zhoumai 27 has the strongest resistance while the resistance of Baoyan 85 is the weakest.

关 键 词：小麦 叶片 丙二醛 过氧化物酶 超氧化物歧化酶 

分 类 号：Q945.79[生物学—植物学]