OPN基因启动区-156delG多态性与中国HBV相关肝细胞癌的易感性  

Osteopontin Promoter Polymorphism at Locus-156 is Associated with HCC in Patients with HBV Infection in Chinese Population

作　　者：时红[1] 吴元凯[1] 林国莉[1] 李向永[1] SHI Hong WU Yuan-kai LIN Guo-li LI Xiang-yong(Department of Infectious Disease, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China)

机构地区：[1]中山大学附属第三医院感染科,广东广州510630

出　　处：《中山大学学报（医学科学版）》2017年第5期717-721,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(20128031800069)

摘　　要：【目的】研究骨桥蛋白(OPN)基因启动区多态性与乙型肝炎病毒相关肝细胞癌(HBV-HCC)遗传易感性的关系。【方法】225例确诊为HBV-HCC患者与200例年龄匹配的慢性乙型肝炎患者被纳入研究。OPN基因启动区3个多态性位点(-156del G/G,-443T/C和-616T/G)通过直接测序法确定基因型。【结果】HBV-HCC组-156 del G/del G基因型分布频率高于对照组(P=0.003)。HCC组-156 del G等位基因频率显著增高(P<0.001)。Logistic回归分析显示等位基因-156del G是HBV-HCC的易感因素(OR=1.643,95%CI 1.249-2.161)。两组间-443T/C、-616T/G基因型分布和等位基因频率差异没有统计学意义。【结论】OPN基因启动区-156等位基因可能是中国人群HBV-HCC的易感因素。【Objective】To study the association between the polymorphisms in the promoter region of Osteopontin（OPN）with hepatitis B virus（HBV）-related HCC. 【Methods】A total of 225 cases diagnosed with hepatitis B virus（HBV）-related HCC and200 age-matched patients with HBV infection without HCC were collected. Three polymorphisms（-156 del G/G,-443T/C and-616T/G）in the Osteopontin promoter were genotyped using direct sequencing.【Results】The frequency of-156 del G/del G genotype in the HCC group was higher than that of in the control group（P = 0.003）. There was a significantly increased frequency of the allele-156 del G（P〈0.001）in HCC patients. Logistic regression analysis was performed to show an increase HCC risk associated with the del G variant genotype（OR1.64;95%CI 1.25 ~ 2.16）. There were no differences between the groups in the genotype distributions and allele frequencies of SNP-443T/C and-616T/G.【Conclusion】Our findings suggest that allele-156 del G in the Osteopontin promoter may be a marker for risk of HCC with HBV infection in Chinese Han population.

关 键 词：骨桥蛋白 基因多态性 肝细胞癌 肝炎 乙型 慢性 

分 类 号：R512.6[医药卫生—内科学] R735.7[医药卫生—临床医学]