复方芒果叶抗炎物质筛选和作用机制研究  

Screening and mechanism of anti-inflammatory ingredients in compound Mango leaves formula

作　　者：刘颖[1] 候小涛 姜民[3] 侯媛媛[3] 张铁军[4] 白钢[3] 邓家刚[1,2] 

机构地区：[1]成都中医药大学,四川成都611137 [2]广西中医药大学,广西中药药效研究重点实验室,农作物废弃物功能成分研究协同创新中心,广西南宁530021 [3]南开大学,天津300071 [4]天津药物研究院,天津300193

出　　处：《中草药》2017年第18期3760-3767,共8页Chinese Traditional and Herbal Drugs

基　　金：广西科技基础条件平台项目(10-046-04);广西“八桂学者”工程专项经费资助(厅发【2013】3号)

摘　　要：目的探究复方芒果叶的抗炎活性,明确其抗炎药效物质基础,初步阐明其网络调控机制。方法采用UPLC-Q/TOF-MS法结合核因子-κB(NF-κB)荧光素酶报告基因检测系统对复方芒果叶进行抗炎成分的筛选;运用网络药理学对其中药效成分进行作用靶点与通路的预测;并利用肿瘤坏死因子-α(TNF-α)诱导人支气管上皮细胞BEAS-2B炎症模型以及采用酶联免疫法(ELISA)对复方芒果叶的抗炎活性进行验证。结果从复方芒果叶中筛选出16个主要抗炎成分;预测这些抗炎成分分别作用于40个靶点并与11条炎症通路相关,活性验证结果表明芒果苷、补骨脂素、人参皂苷Rg1 3个化合物均具有很好的抗炎作用,且各成分间两两配伍具有协同作用。结论初步揭示了复方芒果叶抗炎作用与丝裂原活化蛋白激酶(MAPK)、黏着斑(focal adhesion)、血管内皮生长因子(VEGF)信号通路等多重调控机制有关。Objective To investigate the anti-inflammatory activity of compound Mango leaves formula（CMLF） and illuminate the network regulated mechanism. Methods UPLC-Q/TOF-MS combined with NF-κB dual-luciferase reporter assay systems was used to screen the anti-inflammatory ingredients in CMLF, network pharmacology approach was utilized to forecast the targets and pathways, and then the anti-inflammatory effect was verified by TNF-α induced BEAS-2B cells inflammatory model and ELISA. Results Sixteen anti-inflammatory ingredients were screened out, and there were 40 targets working on 11 pathways respectively. Conclusion The anti-inflammatory multiple regulation mechanism of CMLF is related to MAPK, focal adhesion.

关 键 词：复方芒果叶 抗炎 谱效关系 网络药理学 芒果苷 补骨脂素 人参皂苷RG1 

分 类 号：R285.5[医药卫生—中药学]