酸枣仁汤对老年慢性睡眠剥夺大鼠视交叉上核中血管活性肠肽、精氨酸加压素肽能神经细胞的影响  

Suanzaoren Decoction on Suprachiasmatic Nucleus VIP and AVP Peptide Nerve Cells of Senile Rats with Chronic Sleep Deprivation

作　　者：张如意[1] 游秋云[1] 丁莉[1] 王平[1] 张舜波[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065

出　　处：《辽宁中医杂志》2017年第9期1987-1990,I0006,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473560)

摘　　要：目的:研究酸枣仁汤对老年慢性睡眠剥夺大鼠视交叉上核中血管活性肠肽(VIP)、精氨酸加压素(AVP)肽能神经细胞的影响。方法:利用"多平台水环境法"建立老年大鼠慢性睡眠剥夺模型,将实验大鼠随机分为4组,环境对照组,慢性睡眠剥夺模型组,舒乐安定组以及酸枣仁汤组。用免疫组织化学方法观察睡眠剥夺21 d后各组大鼠视交叉上核中VIP、AVP肽能神经细胞的变化,用Real Time-PCR法检测视交叉上核中VIP mRNA、AVP mRNA的表达。结果:与环境对照组相比较,慢性睡眠剥夺模型组VIP表达明显降低、AVP表达明显升高(P<0.05);与慢性睡眠剥夺模型组比较,治疗组VIP则有不同程度的升高(P<0.05),AVP则出现明显下降,其中酸枣仁汤组上下浮动最为显著。与环境对照组比较,慢性睡眠剥夺模型组VIP、AVPmRNA表达下调;与慢性睡眠剥夺模型组比较,舒乐安定组和酸枣仁汤组VIP、AVPmRNA表达上调。结论:酸枣仁汤能够调控大脑视交叉上核VIP、AVP的表达,有效调节老年睡眠剥夺大鼠的昼夜节律。Objective： To study the influence of Suanzaoren Decoction on suprachiasmatic nucleus VIP and AVP of elderly rats wtih chronic sleep deprivation. Methods： Use the multi-platform water environmental law to set up the rats with chronic sleep deprivation model. The experimental rats were randomly divided into 4 groups： environmental control,chronic sleep deprivation model group,estazolam group and Suanzaoren Decoction group. Use immunohistochemical method to observe sleep deprivation after 21 days between groups of rats in the suprachiasmatic nucleus VIP and AVP peptides nerve cells. Use Real Time PCR method to detect VIP mRNA in the suprachiasmatic nucleus and AVP mRNA expression. Results： Compared with the environment in the control group,chronic sleep deprivation model group＇s VIP expression significantly decreased and AVP increased significantly（ P〈0. 05）. Compared with chronic sleep deprivation model group,treatment group＇ s VIP had varying degrees of elevation（ P〈0. 05）,significant lower in AVP and most dramatic fluctuations were in Suanzaoren Decoction group. Compared with the control group,the expressions of VIP and AVPmRNA in chronic sleep deprivation model were down-regulated. Compared with the chronic sleep deprivation model group,the expressions of VIP and AVPmRNA in the group were raised. Conclusion： Suanzaoren Decoction can control the brain suprachiasmatic nucleus VIP,the expression of AVP and effectively regulate the circadian rhythm of elderly rats with sleep deprivation.

关 键 词：酸枣仁汤 睡眠剥夺 VIP AVP 免疫组化 Real Time-PCR 

分 类 号：R285[医药卫生—中药学]