基于古代本草记载的不同产地艾叶中棕矢车菊素和异泽兰黄素的含量研究  

Quantative analysis of eupatilin and jaceosidin in folium of Artemisia argyi from different areas in China by RP-HPLC based on ancient medicine books

作　　者：黄显章 康利平[1] 高丽[3] 张元[1] 郭兰萍[1] 黄璐琦[1] 

机构地区：[1]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700 [2]中国中医科学院博士后科研流动站,北京100700 [3]南阳理工学院河南省张仲景方药与免疫调节重点实验室,河南南阳473004

出　　处：《中国中药杂志》2017年第18期3504-3508,共5页China Journal of Chinese Materia Medica

基　　金：中央本级重大增减支项目(2060302);国家自然科学基金重点项目(81130070);江苏高校协同创新项目;国家重点基础研究发展计划(973)项目(2015CB554506);河南省科技开放合作项目(172106000053)

摘　　要：为了评价"海艾"、"蕲艾"、"北艾"、"祁艾"和南阳艾叶品质,利用高效液相色谱法同时测定艾叶中棕矢车菊素和异泽兰黄素的含量。采用Agilent Eclipse XDB-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.2%磷酸溶液(35∶65),流速1.0 mL·min^(-1),检测波长350 nm,柱温25℃。棕矢车菊素和异泽兰黄素分别在0.003~0.126 g·L^(-1)(r=0.999 9),0.005~0.200 g·L^(-1)(r=0.999 9)呈良好的线性关系,平均回收率(n=6)分别为99.14%(RSD 1.2%),99.40%(RSD0.73%),说明该方法准确可靠、重复性好,是用于艾叶的质量控制的有效方法。结果表明不同产地艾叶中棕矢车菊素含量差异较小,不同产地艾叶中异泽兰黄素的含量差异较大,淮河以南的浙江宁波、湖北蕲春、湖北襄阳、河南南阳的艾叶和淮河以北汤阴艾叶、安国艾叶中异泽兰黄素含量相对较高。In order to evaluate the quality of Artemisia argyi from Qichun, Ningbo, Anguo and Nanyang, the contents of eupatilin and jaceosidin were determined by RP-HPLC. The determination was performed on Agilent Eclipse XDB-C18 （4.6 mm×250 mm, 5 μm） with mobile phase consisted of acetonitrile-0.2% phosphoric acid（35：65） at the flow rate 1.0 mL·min-1. The detection wavelength was 350 nm and the column temperature was 25℃. The results showed that the amount of eupatilin and jaceosidin had a clear linear relationship in the range of 0.003-0.126 g·L-1 （r=0.999 9） and 0.005-0.200 g·L-1 （r=0.999 9）, and the average recovery rates for them were 99.14% （n=6, RSD 1.2%） and 99.40% （n=6, RSD=0.73%）, respectively. The results showed that RP-HPLC can be used for the quantification of eupatilin and jaceosidin in the folium of A. argyi. With this method, we found there was no significant difference of jaceosidin content within all the samples collected, but the content of eupatilin was significantly higher in samples from Qichun, Ningbo, Xiangyang and Nanyang, located in the south of Huaihe River compared with these from other areas.

关 键 词：艾叶 棕矢车菊素 异泽兰黄素 含量研究 

分 类 号：R284.1[医药卫生—中药学]