大鼠肠道菌群对羊耳菊提取物的代谢作用研究  

Metabolism of Inula cappa extract by rat intestinal bacteria in vitro

作　　者：巩仔鹏[1,2,3,4] 侯靖宇 李梅[1,2,3,4] 吴林霖 陈亭亭[1,2,3,4] 李勇军 王爱民 兰燕宇 王永林[1,2,3,4] 

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室,贵州贵阳550004 [2]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [3]贵州医科大学药学院,贵州贵阳550004 [4]国家苗药工程研究中心,贵州贵阳550004

出　　处：《中国中药杂志》2017年第18期3584-3590,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81360680);贵州省优青项目(黔科合人字[2015]11号);贵州省教育厅项目(黔科合KY字[2013]122);贵州省科技合作计划项目(黔科合LH字[2015]7362);贵州省研究生卓越人才计划项目(黔教研合ZYRC字[2014]012);贵州省中医药管理局项目(QZZY-2015-078)

摘　　要：考察大鼠肠道菌群对羊耳菊提取物中主要成分的代谢作用,将羊耳菊提取物与大鼠肠道菌液在厌氧条件下共同孵育24 h,通过正丁醇液液萃取处理后,采用色谱联用技术(UPLC-Q-TOF-MS/MS)对代谢产物进行定性分析,并结合布鲁克公司研发的数据处理工具Metabolite Tools,Data Analysis等对代谢信息进行综合分析。通过比较空白样品图谱,肠菌样品图谱以及两者的差异图谱,各色谱峰的准分子离子,碎片离子,分析羊耳菊提取物在大鼠肠道菌群作用下可能产生的代谢产物。结果提示,羊耳菊提取物在肠道菌群作用下,检测到14个代谢产物,主要包括单咖啡酰基奎宁酸的异构,水解的小分子化合物和咖啡酸的还原,甲基化和乙酰化等代谢产物,同时仅检测到1个二咖啡酰基奎宁酸的甲基化代谢产物。以此推测,咖啡酰基奎宁酸类成分在肠道菌群的作用下可能多数水解为分子量小,疏水性更强的代谢产物,使得其更容易被肠道吸收。To investigate the metabolism of major components in Inula cappa by rat intestinal bacteria in vitro. I. cappa extract was incubated for 24 h with rat intestinal bacteria under anaerobic environment. After the samples were precipitated by n-butanol, UPLC-Q- TOF-MS/MS was applied for the qualitative analysis of the metabolites, combined with data software such as Metabolite Tools, Data Analysis and so on. The potential metaboiites in rat intestinal bacteria were analyzed by comparing the total ion current of the test sam- ples and blank samples and analyzing the quasi-molecular ion and fragment ion of all chromatograms. The results injected that fourteen metabolites were detected in rat intestinal flora. Various types of metabolic reactions happen to caffeoylquinic acid in intestinal flora,including isomerization, hydrolyzation, there were also methylation, hydrogenation and acetylation of caffeic acid. At the same time, a methylate of dicaffeoylquinic acid was also detected. Presumably, caffeoylquinic acids were gradually transformed into more hydropho- bic metabolites with smaller molecular mass, which were better absorbed by the intestinal tract.

关 键 词：羊耳菊提取物 肠道菌群 代谢产物 UPLC-Q-TOF-MS/MS 

分 类 号：R285.5[医药卫生—中药学]