一种用于活细胞中检测Zn^(2+)的萘酚席夫碱类荧光探针（英文）  

Naphthol-Based Shiff Base as a Selective Fluorescent Probe for Detecting Zn^(2+) in Living Cells

作　　者：陈邦[1] 王少静[1] 宋战科 郭媛[1] 

机构地区：[1]西北大学化学与材料科学学院合成与天然功能分子化学教育部重点实验室,西安710127

出　　处：《无机化学学报》2017年第10期1722-1730,共9页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21472148,21072158);陕西省留学人员科技活动择优资助项目(No.20151190);西北大学优秀青年学术骨干支持计划资助

摘　　要：设计合成了1种基于C=N异构化和螯合荧光增强机理(CHEF)的Zn^(2+)荧光探针BMO和NBMO,其结构经~1H NMR,^(13)C NMR,~1H-~1H COSY,HSQC,IR和HRMS进行了表征。光谱分析实验结果显示,探针对Zn^(2+)均具有较好的选择性和灵敏度,检出限分别为30和21 nmol·L^(-1)。在0~20μmol·L^(-1)浓度的范围内,BMO和NBMO的荧光强度与Zn^(2+)浓度可呈良好的线性关系。NBMOZn^(2+)配合物单晶结构和Job曲线证实该探针与Zn^(2+)以1∶1配位。NBMO被成功应用于活细胞中Zn^(2+)的检测。Two fluorescent probes BMO and NBMO were developed for the highly selective detection of Zn2＋ based on C=N isomerization and chelation-enhanced fluorescence （CHEF） mechanism. Structure identification of the probes was confirmed by 1H NMR, 13C NMR, 1H-1H COSY, HSQC, IR and HRMS spectroscopy. The results of spectral analysis show that BMO and NBMO are sensitive and highly selective to Zn2＋. The detection limit of BMO and NBMO are found to be 30 and 21 nmol·L-1 respectively. There is a good linear relationship between the fluorescence intensity of probes （BMO and NBMO） and the concentration of Zn2＋ in the range of 0 to 20 μmol·L-1. The X-ray crystal structure of the NBMO-Zn2＋ complex exposes its coordination feature and the Job plots reveal a 1：1 probe-Zn2＋ identification. The cell images show that NBMO can be used to detect intracellular Zn2＋.

关 键 词：荧光探针 C=N异构化 螯合荧光增强 锌离子 活细胞成像 

分 类 号：O626.23[理学—有机化学]