原核生物全基因组中16S rRNA基因的识别  

Recognition of 16S rRNA genes in prokaryotic genomes

作　　者：闫文凯 许明敏 张广乐 乔宁 徐炜娜 陈园园[1] 张良云[1] Wenkai Yan Mingmin Xu Guangle Zhang Ning Qiao Weina Xu Yuanyuan Chen Liangyun Zhang(College of Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China)

机构地区：[1]南京农业大学理学院,江苏南京210095

出　　处：《微生物学报》2017年第10期1493-1503,共11页Acta Microbiologica Sinica

基　　金：国家自然科学基金(11571173);江苏省自然科学基金(BK20141358)~~

摘　　要：【目的】识别原核生物全基因组中的16S rRNA基因。【方法】本文依据基因序列的GC碱基含量、碱基3-周期性和马尔可夫链3个方面的特性,构建了识别原核生物全基因组中16S rRNA基因的三层过滤模型。【结果】经检验,模型的特异性、敏感性和马修斯相关系数分别为99.58%、91.60%和91.49%。【结论】结果表明,本文所提出的方法可以高效、准确地识别出16S rRNA基因。[Objective] We identified 16S rRNA genes in genomes of prokaryotes. [Methods] We constructed a 3-layer filtering model based on the three features of GC bases content of the gene sequences, 3-base periodicity and Markov chain to recognize the 16S rRNA genes from prokaryotic genomes. [Results] The specificity, sensitivity and Mat-thews correlation coefficients of the model were 99.58%, 91.60% and 91.49%, respectively. [Conclusion] The results showed that the 16S rRNA genes can be identified efficiently and accurately by using our model.

关 键 词：16S RRNA基因 GC碱基含量 碱基3-周期性 马尔可夫链 

分 类 号：Q78[生物学—分子生物学]