血清白介素-27水平与视神经脊髓炎患者疾病严重程度的关系  

The study on the association of serum interleukin-27 levels with disease severity in neuromyelitis optica

作　　者：贾昆[1] 刘小姣 周冰洁 张大启 杨丽 

机构地区：[1]天津医科大学总医院、天津市神经病学研究所 [2]天津市环湖医院,天津300350

出　　处：《中国神经精神疾病杂志》2017年第8期480-484,共5页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金面上项目(编号:81471221);天津市应用基础与前沿技术研究计划(重点项目)(编号:15JCZDJC35700)

摘　　要：目的检测视神经脊髓炎(neuromyelitis optica,NMO)患者与健康人血清中白介素27(interleukin-27,IL-27)水平,探讨其在NMO发病中的意义及其与疾病严重程度的相关性。方法采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测39例NMO急性期患者与39例健康志愿者血清中IL-27水平,比较组间差异及分析NMO组血清IL-27水平与扩展残疾状况评分(expanded disability status scale,EDSS)、脊髓受累节段数、年复发率及水通道蛋白4(aquaporin-4,AQP-4)抗体滴度等临床指标的相关性。结果 NMO组血清IL-27水平较健康对照组(healthy controls,HCs)明显降低,差异具有统计学意义(P<0.001),且NMO组患者血清IL-27水平与EDSS评分、脊髓受累节段数、随访2年平均复发率呈负相关(r=-0.439,P=0.010;r=-0.434,P=0.006;r=-0.451,P=0.031),而与AQP-4抗体滴度不存在相关性(r=-0.027,P=0.871)。多元回归分析显示血清IL-27水平与EDSS评分存在负相关性(B=-0.025,P=0.023)。结论 IL-27可能参与了NMO的发病过程,且与疾病严重程度负相关,同时对于疾病的预后具有一定影响。Objective To examine the interleukin-27（IL-27） levels in the serum of the patients with neuromyelitis optica（NMO） and healthy controls（HCs） and explore the correlation of the serum IL-27 level with disease severity. Methods Serum concentrations of IL-27 from 39 patients with NMO and 39 HCs were measured by using enzyme-linked immunosorbent assay（ELISA）. Mann-Whitney U test was performed to analyze the difference in the IL-27 levels between the NMO group and the HCs. Spearman＇s rank correlation analysis was used to analyze the correlations of serum IL-27 levels with clinical parameters（EDSS, spinal cord lesion length, annual relapse rate and antibody titers of AQP-4） of NMO. Results The serum IL-27 levels were significantly lower in NMO group than in HCs（P〈0.001）. Serum IL-27 levels were negatively correlated with EDSS, total length of spinal cord lesion identified by MRI at the sampling,and the average relapse rate during two-year follow-up（r=-0.439, P=0.010; r=-0.434, P=0.006; r=-0.451, P=0.031）.There was no significant correlation between IL-27 levels and antibody titers of AQP-4（r=-0.027, P=0.871）. Multivariate regression analysis showed that serum IL-27 levels were negatively correlated with EDSS（B =-0.025,P =0.023）.Conclusion IL-27 may participate in the pathogenic process of NMO and might be a prognosis marker of the disease.

关 键 词：视神经脊髓炎 白介素-27 酶联免疫吸附法 水通道蛋白-4抗体 

分 类 号：R744.52[医药卫生—神经病学与精神病学]